First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands

Bastian, Sylvaine; Nordmann, Patrice; Creton, Elodie; Malpote, Edith; Thiery, Guillaume; Martino, Frederic; Breurec, Sebastien; Dortet, Laurent

doi:10.1016/j.ijid.2015.03.002

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Journal article

First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands

Bastian, Sylvaine University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France
Nordmann, Patrice INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France - Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France - University of Fribourg, Fribourg, Switzerland - Hôpital Hôpital Cantonal, Fribourg, Switzerland
Creton, Elodie INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France - Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France
Malpote, Edith University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France
Thiery, Guillaume University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France - University of Antilles, Pointe-à-Pitre, Guadeloupe, France
Martino, Frederic University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France
Breurec, Sebastien University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France - University of Antilles, Pointe-à-Pitre, Guadeloupe, France
Dortet, Laurent INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France - Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France - South Paris University, Faculty of Medecine, Le Kremlin-Bicêtre, France - Assistance Publique des Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France

05.03.2015

Published in:

International Journal of Infectious Diseases. - 2015, vol. 34, p. 53–54

English Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from CubaMethodsAntibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were characterized by PCR mapping and DNA sequencing. Transfer of the β-lactam resistance marker was attempted by liquid mating-out assaysResultsHere we reported the first NDM-1 producing enterobacterial isolate recovered from Caribbean islands. This K. pneumoniae isolate belongs to a new sequence type (ST1649). The bla_NDM-1 gene together with the aacA4 gene were carried on a self conjugative IncR plasmid of c.a. 80 kb.ConclusionThis study describes the first identification of a NDM-1 producer in Caribbean islands. The uncommon incompatibility group of the bla_NDM-1 carrying plasmid and the uncommon ST type of the K. pneumoniae strain suggest a possible local emergence of NDM producers.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language