Bastian, Sylvaine Nordmann, Patrice Creton, Elodie Malpote, Edith Thiery, Guillaume Martino, Frederic Breurec, Sebastien Dortet, Laurent 2015-03-05 Characterize a NDM-1 producing <em>K. pneumoniae</em> isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from CubaMethodsAntibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were characterized by PCR mapping and DNA sequencing. Transfer of the β-lactam resistance marker was attempted by liquid mating-out assaysResultsHere we reported the first NDM-1 producing enterobacterial isolate recovered from Caribbean islands. This <em>K. pneumoniae</em> isolate belongs to a new sequence type (ST1649). The <em>bla</em>_NDM-1 gene together with the <em>aacA4</em> gene were carried on a self conjugative IncR plasmid of c.a. 80 kb.ConclusionThis study describes the first identification of a NDM-1 producer in Caribbean islands. The uncommon incompatibility group of the <em>bla</em>_NDM-1 carrying plasmid and the uncommon ST type of the <em>K. pneumoniae</em> strain suggest a possible local emergence of NDM producers. application/pdf https://folia.unifr.ch/global/documents/304593 https://folia.unifr.ch/documents/304593/files/nor_fcn.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijid.2015.03.002 info:eu-repo/semantics/openAccess License undefined International Journal of Infectious Diseases. - 2015, vol. 34, p. 53–54 NDM-1 Carbapenemase Metallo-β-lactamase Cuba Guadeloupe info:eu-repo/classification/udc/57 First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands http://purl.org/coar/resource_type/c_6501