Journal article

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Antibodies and IL-3 support helminth-induced basophil expansion

  • Herbst, Tina Institute of Microbiology, Swiss Federal Institute of Technology, Zurich, Switzerland
  • Esser, Julia Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
  • Prati, Moira Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
  • Kulagin, Manuel Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
  • Stettler, Rebecca Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
  • Zaiss, Mario M. Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
  • Hewitson, James P. Institute of Immunology and Infection Research, University of Edinburgh, United Kingdom
  • Merky, Patrick Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
  • Verbeek, Joseph S. Department of Human Genetics, Leiden University Medical Center, The Netherlands
  • Bourquin, Carole Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
  • Camberis, Mali Malaghan Institute of Medical Research, Wellington, New Zealand
  • Prout, Melanie Malaghan Institute of Medical Research, Wellington, New Zealand
  • Maizels, Rick M. Institute of Immunology and Infection Research, University of Edinburgh, United Kingdom
  • Gros, Graham Le Malaghan Institute of Medical Research, Wellington, New Zealand
  • Harris, Nicola L. Swiss Vaccine Research Institute and Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Switzerland
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    28.08.2012
Published in:
  • Proceedings of the National Academy of Sciences of the United States of America. - 2012, vol. 109, no. 37, p. 14954-14959
English Basophils are powerful mediators of Th2 immunity and are present in increased numbers during allergic inflammation and helminth infection. Despite their ability to potentiate Th2 immunity the mechanisms regulating basophil development remain largely unknown. We have found a unique role for isotype-switched antibodies in promoting helminth-induced basophil production following infection of mice with Heligmosomoides polygyrus bakeri or Nippostrongylus brasiliensis. H. polygyrus bakeri-induced basophil expansion was found to occur within the bone marrow, and to a lesser extent the spleen, and was IL-3 dependent. IL-3 was largely produced by CD4+CD49b+NK1.1− effector T cells at these sites, and required the IL-4Rα chain. However, antibody-deficient mice exhibited defective basophil mobilization despite intact T-cell IL-3 production, and supplementation of mice with immune serum could promote basophilia independently of required IL-4Rα signaling. Helminth-induced eosinophilia was not affected by the deficiency in isotype-switched antibodies, suggesting a direct effect on basophils rather than through priming of Th2 responses. Although normal type 2 immunity occurred in the basopenic mice following primary infection with H. polygyrus bakeri, parasite rejection following challenge infection was impaired. These data reveal a role for isotype-switched antibodies in promoting basophil expansion and effector function following helminth infection.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biology
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/302803
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