Rapid ESBL NP test for rapid detection of expanded-spectrum β-lactamase producers in enterobacterales
- Demord, Anthony Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
- Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland.
- D'Emidio, Fernando Liofilchem, Roseto degli Abruzzi, Italy.
- Pomponio, Stefano Liofilchem, Roseto degli Abruzzi, Italy.
- Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland. - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
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10.12.2020
Published in:
- Microbial Drug Resistance. - 2020, vol. 27, no. 8, p. 1131-1135
English
Objectives: To evaluate the biochemical Rapid ESBL NP® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.Methods: A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique.Results: The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40–45 minutes), easy to perform, and reliable for identification of ESBL producers.Conclusion: The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 329950
- DOI 10.1089/mdr.2020.0391
- Persistent URL
- https://folia.unifr.ch/unifr/documents/309119
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