Rapid ESBL NP test for rapid detection of expanded-spectrum β-lactamase producers in enterobacterales

Demord, Anthony; Poirel, Laurent; D'Emidio, Fernando; Pomponio, Stefano; Nordmann, Patrice

doi:10.1089/mdr.2020.0391

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Journal article

Rapid ESBL NP test for rapid detection of expanded-spectrum β-lactamase producers in enterobacterales

Demord, Anthony Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland.
D'Emidio, Fernando Liofilchem, Roseto degli Abruzzi, Italy.
Pomponio, Stefano Liofilchem, Roseto degli Abruzzi, Italy.
Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland. - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.

10.12.2020

Published in:

Microbial Drug Resistance. - 2020, vol. 27, no. 8, p. 1131-1135

English Objectives: To evaluate the biochemical Rapid ESBL NP® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.Methods: A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique.Results: The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40–45 minutes), easy to perform, and reliable for identification of ESBL producers.Conclusion: The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language