Journal article

Phenotypic, biochemical, and genetic analysis of kpc-41, a kpc-3 variant conferring resistance to ceftazidime-avibactam and exhibiting reduced carbapenemase activity

  • Mueller, Linda Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - Institut for Microbiology, University Hospital Center and University of Lausanne, Switzerland
  • Masseron, Amandine Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland
  • Prod’Hom, Guy Institut for Microbiology, University Hospital Center and University of Lausanne, Switzerland
  • Galperine, Tatiana Infectiology Department, University Hospital Center and University of Lausanne, Switzerland
  • Greub, Gilbert Institut for Microbiology, University Hospital Center and University of Lausanne, Switzerland
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - Institut for Microbiology, University Hospital Center and University of Lausanne, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland
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    01.12.2019
Published in:
  • Antimicrobial Agents and Chemotherapy. - 2019, vol. 63, no. 12
English A novel KPC variant, KPC-41, was identified in a Klebsiella pneumoniae clinical isolate from Switzerland. This β-lactamase possessed a 3-amino-acid insertion (Pro- Asn-Lys) located between amino acids 269 and 270 compared to the KPC-3 amino acid sequence. Cloning and expression of the blaKPC-41 gene in Escherichia coli, followed by determination of MIC values and kinetic parameters, showed that KPC-41, compared to those of KPC-3, has an increased affinity to ceftazidime and a decreased sensitivity to avibactam, leading to resistance to ceftazidime-avibactam once produced in K. pneumoniae. Furthermore, KPC-41 exhibited a drastic decrease of its carbapenemase activity. This report highlights that a diversity of KPC variants conferring resistance to ceftazidime-avibactam already circulate in Europe.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308401
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