Phosphate systemically inhibits development of arbuscular mycorrhiza in Petunia hybrida and represses genes involved in mycorrhizal functioning

Breuillin, Florence; Schramm, Jonathan; Hajirezaei, Mohammad; Ahkami, Amir; Favre, Patrick; Druege, Uwe; Hause, Bettina; Bucher, Marcel; Kretzschmar, Tobias; Bossolini, Eligio; Kuhlemeier, Cris; Martinoia, Enrico; Franken, Philipp; Scholz, Uwe; Reinhardt, Didier

doi:10.1111/j.1365-313X.2010.04385.x

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Phosphate systemically inhibits development of arbuscular mycorrhiza in Petunia hybrida and represses genes involved in mycorrhizal functioning

Breuillin, Florence Department of Biology, University of Fribourg, Switzerland - Boyce Thompson Institute for Plant Research, Ithaca, USA
Schramm, Jonathan Department of Biology, University of Fribourg, Switzerland
Hajirezaei, Mohammad Leibniz-Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
Ahkami, Amir Leibniz-Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
Favre, Patrick Department of Biology, University of Fribourg, Switzerland
Druege, Uwe Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany
Hause, Bettina Leibniz-Institute of Plant Biochemistry, Halle, Germany
Bucher, Marcel University of Cologne, Botanical Institute, Köln, Germany
Kretzschmar, Tobias Institute of Plant Biology, University of Zürich, Switzerland
Bossolini, Eligio University of Berne, IPS, Switzerland
Kuhlemeier, Cris University of Berne, IPS, Switzerland
Martinoia, Enrico Institute of Plant Biology, University of Zürich, Switzerland
Franken, Philipp Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany
Scholz, Uwe Leibniz-Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
Reinhardt, Didier Department of Biology, University of Fribourg, Switzerland

04.11.2010

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The Plant Journal. - 2010, vol. 64, no. 6, p. 1002-1017

English Most terrestrial plants form arbuscular mycorrhiza (AM), mutualistic associations with soil fungi of the order Glomeromycota. The obligate biotrophic fungi trade mineral nutrients, mainly phosphate (P_i), for carbohydrates from the plants. Under conditions of high exogenous phosphate supply, when the plant can meet its own P requirements without the fungus, AM are suppressed, an effect which could be interpreted as an active strategy of the plant to limit carbohydrate consumption of the fungus by inhibiting its proliferation in the roots. However, the mechanisms involved in fungal inhibition are poorly understood. Here, we employ a transcriptomic approach to get insight into potential shifts in metabolic activity and symbiotic signalling, and in the defence status of plants exposed to high P_i levels. We show that in mycorrhizal roots of petunia, a similar set of symbiosis-related genes is expressed as in mycorrhizal roots of Medicago, Lotus and rice. P_i acts systemically to repress symbiotic gene expression and AM colonization in the root. In established mycorrhizal roots, P_i repressed symbiotic gene expression rapidly, whereas the inhibition of colonization followed with a lag of more than a week. Taken together, these results suggest that P_i acts by repressing essential symbiotic genes, in particular genes encoding enzymes of carotenoid and strigolactone biosynthesis, and symbiosis-associated phosphate transporters. The role of these effects in the suppression of symbiosis under high P_i conditions is discussed.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language