Fluorometric toolkit for methanethiol quantification and methanethiol oxidase activity determination in biological systems

Philipp, Thilo M.; Schiffmann, Belen; Tintrop, Lucie K.; Findlay, Jacqueline; Krafczyk, Niklas; Schlemminger, Hanna; Ainsa-Zazurca, Susana; Ferreira, Vicente; Fuchsmann, Pascal; Nordmann, Patrice; Klotz, Lars-Oliver; Szabo, Csaba; Majtan, Tomas

doi:10.1016/j.redox.2026.104288

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2026philippmethanethiolmtofluorescentassaytoolkit

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Fluorometric toolkit for methanethiol quantification and methanethiol oxidase activity determination in biological systems

DOKPE

Philipp, Thilo M. University of Fribourg
Schiffmann, Belen University of Fribourg
Tintrop, Lucie K. Agroscope, Bern
Findlay, Jacqueline University of Fribourg
Krafczyk, Niklas Friedrich Schiller University Jena
Schlemminger, Hanna Friedrich Schiller University Jena
Ainsa-Zazurca, Susana University of Zaragoza
Ferreira, Vicente University of Zaragoza
Fuchsmann, Pascal Agroscope, Bern
Nordmann, Patrice University of Fribourg
Klotz, Lars-Oliver Friedrich Schiller University Jena
Szabo, Csaba University of Fribourg
Majtan, Tomas ORCID University of Fribourg

2026

Published in:

Redox Biology. - Elsevier BV. - 2026, vol. 95, p. 104288

English Methanethiol (MeSH) is a pivotal volatile sulfur compound in plant, human, and environmental chemistry, but its study is hindered by analytical challenges. Here, we present an accessible fluorometric two-component assay toolkit for quantitative assessment of its content and enzymatic degradation by methanethiol oxidase (MTO). The first assay sensitively detects MeSH with a limit of detection of 124 nM using a novel “tube-in-tube” design for efficient MeSH capture, circumventing the need for complex chromatography and expensive instrumentation. The second assay is a real-time kinetic MTO activity assay. Using this toolkit, we (i) established an inverse correlation between MTO activity and MeSH levels in a colon cancer cell line, (ii) quantified MeSH as a key scent marker in various types of cheese, (iii) evaluated MeSH release from the selected Brassica and Allium species and
(iv) determined its production in aquatic systems. The MeSH metabolism toolkit presented here will provide researchers with simple, sensitive and specific assays to elucidate multifaceted roles of MeSH and MTO in human pathophysiology and to accelerate discoveries in a broad spectrum of scientific disciplines.

Faculty

Faculté des sciences et de médecine

Department

Section de médecine

Language