Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs
- Manganelli, Valeria Department of Experimental Medicine, Sapienza University , Rome, Italy
- Matarrese, Paola Oncology Unit, Center for Gender-Specific Medicine, Istituto Superiore di Sanità , Rome, Italy
- Antonioli, Manuela Department of Epidemiology, Preclinical Research and Advanced Diagnostics, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS , Rome, Italy
- Gambardella, Lucrezia Oncology Unit, Center for Gender-Specific Medicine, Istituto Superiore di Sanità , Rome, Italy
- Vescovo, Tiziana Department of Epidemiology, Preclinical Research and Advanced Diagnostics, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS , Rome, Italy
- Gretzmeier, Christine Department of Dermatology, Faculty of Medicine, Medical Center-University of Freiburg , Freiburg, Germany
- Longo, Agostina Department of Experimental Medicine, Sapienza University , Rome, Italy
- Capozzi, Antonella Department of Experimental Medicine, Sapienza University , Rome, Italy
- Recalchi, Serena Department of Experimental Medicine, Sapienza University , Rome, Italy
- Riitano, Gloria Department of Experimental Medicine, Sapienza University , Rome, Italy
- Misasi, Roberta Department of Experimental Medicine, Sapienza University , Rome, Italy
- Dengjel, Jörn Department of Dermatology, Faculty of Medicine, Medical Center-University of Freiburg , Freiburg, Germany - Department of Biology, University of Fribourg , Suisse, Germany
- Malorni, Walter School of Pharmacy, University of Tor Vergata , Rome, Italy
- Fimia, Gian Maria Department of Epidemiology, Preclinical Research and Advanced Diagnostics, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS , Rome, Italy - Department of Molecular Medicine, Sapienza University , Rome, Italy
- Sorice, Maurizio Department of Experimental Medicine, Sapienza University , Rome, Italy
- Garofalo, Tina Department of Experimental Medicine, Sapienza University , Rome, Italy
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09.10.2020
Published in:
- Autophagy. - 2020, p. 1-21
English
Mitochondria-associated membranes (MAMs) are essential communication subdomains of the endoplasmic reticulum (ER) that interact with mitochondria. We previously demonstrated that, upon macroautophagy/autophagy induction, AMBRA1 is recruited to the BECN1 complex and relocalizes to MAMs, where it regulates autophagy by interacting with raft-like components. ERLIN1 is an endoplasmic reticulum lipid raft protein of the prohibitin family. However, little is known about its association with the MAM interface and its involvement in autophagic initiation. In this study, we investigated ERLIN1 association with MAM raft-like microdomains and its interaction with AMBRA1 in the regulation of the autophagic process. We show that ERLIN1 interacts with AMBRA1 at MAM raft-like microdomains, which represents an essential condition for autophagosome formation upon nutrient starvation, as demonstrated by knocking down ERLIN1 gene expression. Moreover, this interaction depends on the “integrity” of key molecules, such as ganglioside GD3 and MFN2. Indeed, knocking down ST8SIA1/GD3-synthase or MFN2 expression impairs AMBRA1-ERLIN1 interaction at the MAM level and hinders autophagy. In conclusion, AMBRA1-ERLIN1 interaction within MAM raft-like microdomains appears to be pivotal in promoting the formation of autophagosomes.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 329725
- DOI 10.1080/15548627.2020.1834207
- Persistent URL
- https://folia.unifr.ch/unifr/documents/309060
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