High-risk KPC-producing Klebsiella pneumoniae lack type I R-M systems

Zhou, Ying; Tian, Dongxing; Tang, Yu; Yu, Lianhua; Huang, Yunkun; Li, Gang; Li, Meng; Wang, Yong; Yang, Zehua; Poirel, Laurent; Jiang, Xiaofei

doi:10.1016/j.ijantimicag.2020.106050

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High-risk KPC-producing Klebsiella pneumoniae lack type I R-M systems

Zhou, Ying Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Tian, Dongxing Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Tang, Yu Department of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China
Yu, Lianhua Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China
Huang, Yunkun Department of Laboratory Medicine Kunming Yan'an Hospital, Kunming, China
Li, Gang Department of Laboratory Medicine, Jinshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Li, Meng Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China
Wang, Yong Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, China
Yang, Zehua Department of Laboratory Medicine, Sixth Hospital of Shanxi Medical University, Taiyuan, China
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland - Laboratoire Europeen ASSOCIE (LEA) INSERH, IAME (Paris, France), University of Fribourg, Fribourg, Switzerland
Jiang, Xiaofei Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China

01.08.2020

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International Journal of Antimicrobial Agents. - 2020, vol. 56, no. 2, p. 106050

English Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and are a major threat to public health. The multidrug- resistant (MDR)-phenotype of KPC-KP are commonly associated with the presence of high molecular weight blaKPC plasmids. Restriction-modification (R-M) systems provide bacteria with innate defense against plasmids or other infectious gene elements. As blaKPC plasmids are favored by such MDR K. pneumoniae, it was of interest to examine the co-distribution of R-M and acquired blaKPC plasmids in KPC- KP. A total of 459 clinical K. pneumoniae isolates in China and 217 global whole- genome sequences in GenBank were collected to determine the prevalence of type I R-M systems. The type I R-M systems were scarce in the KPC-positive group and high-risk Klebsiella pneumoniae clonal group 258 (CG258). The polymorphisms of type I R-M observed in K. pneumoniae revealed the ubiquity of their recognition sequences in DNA; therefore, the type I R-M systems could attack most invading DNA elements, such as blaKPC genes. Overall, this work indicated the type I R-M systems may impact the acquisition of blaKPC genes in K. pneumoniae.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language