ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans
- Kieffer, Nicolas Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
- Guzmán-Puche, Julia Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland - University Hospital Reina Sofia, IMIBIC, University of Córdoba, Córdoba, Spain
- Poirel, Laurent Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland
- Kang, Hyo Jung Department of Life Science and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea
- Jeon, Che Ok Department of Life Science and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea
- Nordmann, Patrice Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland
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01.06.2019
Published in:
- Journal of Antimicrobial Chemotherapy. - 2019, vol. 74, no. 6, p. 1568–1571
English
Objectives: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment.Methods: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme.Results: The enzyme named ZHO-1 shared 51% amino acid identity with the acquired class B carbapenemases IMP-1, KHM-1 and DIM-1. Expression of the blaZHO-1 gene in a susceptible E. coli resulted in a carbapenemase phenotype. Kinetic parameters determined from purified ZHO-1 enzyme showed that it had significant hydrolytic activity against most β-lactams including penicillins, cephalosporins and carbapenems, with the exception of aztreonam and cefepime.Conclusions: This study adds to the knowledge regarding environmental species as a reservoir of possible clinically relevant MBLs.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 327453
- DOI 10.1093/jac/dkz057
- Persistent URL
- https://folia.unifr.ch/unifr/documents/308252
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