The auxin influx carrier, OsAUX3, regulates rice root development and responses to aluminium stress

Wang, Mei; Qiao, JiYue; Yu, ChenLiang; Chen, Hao; Sun, ChenDong; Huang, LinZhou; Li, ChuanYou; Geisler, Markus; Qian, Qian; Jiang, De An; Qi, YanHua

doi:10.1111/pce.13478

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The auxin influx carrier, OsAUX3, regulates rice root development and responses to aluminium stress

Wang, Mei State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
Qiao, JiYue State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
Yu, ChenLiang Vegetable Research Institute Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 China
Chen, Hao State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
Sun, ChenDong State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
Huang, LinZhou State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101 China
Li, ChuanYou State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101 China
Geisler, Markus Department of Biology, University of Fribourg, Switzerland
Qian, Qian State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences Hangzhou 310006 China
Jiang, De An State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
Qi, YanHua State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China

2019

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Plant, Cell & Environment. - 2019, vol. 42, no. 4, p. 1125–1138

English In rice, there are five members of the auxin carrier AUXIN1/LIKE AUX1 family; however, the biological functions of the other four members besides OsAUX1 remain unknown. Here, by using CRISPR/Cas9, we constructed two independent OsAUX3 knock‐down lines, osaux3‐1 and osaux3‐2, in wild‐type rice, Hwayoung (WT/HY) and Dongjin (WT/DJ). osaux3‐1 and osaux3‐2 have shorter primary roots (PRs), decreased lateral root (LR) density, and longer root hairs (RHs) compared with their WT. OsAUX3 expression in PRs, LRs, and RHs further supports that OsAUX3 plays a critical role in the regulation of root development. OsAUX3 locates at the plasma membrane and functions as an auxin influx carrier affecting acropetal auxin transport. OsAUX3 is up‐regulated in the root apex under aluminium (Al) stress, and osaux3‐2 is insensitive to Al treatments. Furthermore, 1‐naphthylacetic acid accented the sensitivity of WT/DJ and osaux3‐2 to respond to Al stress. Auxin concentrations, Al contents, and Al‐induced reactive oxygen species‐mediated damage in osaux3‐2 under Al stress are lower than in WT, indicating that OsAUX3 is involved in Al‐induced inhibition of root growth. This study uncovers a novel pathway alleviating Al‐induced oxidative damage by inhibition of acropetal auxin transport and provides a new option for engineering Al‐tolerant rice species.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

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