The auxin influx carrier, OsAUX3, regulates rice root development and responses to aluminium stress
- Wang, Mei State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
- Qiao, JiYue State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
- Yu, ChenLiang Vegetable Research Institute Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 China
- Chen, Hao State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
- Sun, ChenDong State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
- Huang, LinZhou State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101 China
- Li, ChuanYou State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101 China
- Geisler, Markus Department of Biology, University of Fribourg, Switzerland
- Qian, Qian State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences Hangzhou 310006 China
- Jiang, De An State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
- Qi, YanHua State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University Hangzhou 310058 China
-
2019
Published in:
- Plant, Cell & Environment. - 2019, vol. 42, no. 4, p. 1125–1138
English
In rice, there are five members of the auxin carrier AUXIN1/LIKE AUX1 family; however, the biological functions of the other four members besides OsAUX1 remain unknown. Here, by using CRISPR/Cas9, we constructed two independent OsAUX3 knock‐down lines, osaux3‐1 and osaux3‐2, in wild‐type rice, Hwayoung (WT/HY) and Dongjin (WT/DJ). osaux3‐1 and osaux3‐2 have shorter primary roots (PRs), decreased lateral root (LR) density, and longer root hairs (RHs) compared with their WT. OsAUX3 expression in PRs, LRs, and RHs further supports that OsAUX3 plays a critical role in the regulation of root development. OsAUX3 locates at the plasma membrane and functions as an auxin influx carrier affecting acropetal auxin transport. OsAUX3 is up‐regulated in the root apex under aluminium (Al) stress, and osaux3‐2 is insensitive to Al treatments. Furthermore, 1‐naphthylacetic acid accented the sensitivity of WT/DJ and osaux3‐2 to respond to Al stress. Auxin concentrations, Al contents, and Al‐induced reactive oxygen species‐mediated damage in osaux3‐2 under Al stress are lower than in WT, indicating that OsAUX3 is involved in Al‐induced inhibition of root growth. This study uncovers a novel pathway alleviating Al‐induced oxidative damage by inhibition of acropetal auxin transport and provides a new option for engineering Al‐tolerant rice species.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
-
- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
-
- RERO DOC 324631
- DOI 10.1111/pce.13478
- Persistent URL
- https://folia.unifr.ch/unifr/documents/307830
Other files
Statistics
Document views: 55
File downloads:
- gei_aic.pdf: 166
- gei_aic_sm.pdf: 84