Journal article

A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

  • Lescat, Mathilde Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - Université Paris Nord, Sorbonne Paris Cité, Paris, France - Université Paris Nord, Sorbonne Paris Cité, Paris, France
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Paris, France
  • Tinguely, Camille Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - Université Paris Nord, Sorbonne Paris Cité, Paris, France - Université Paris Nord, Sorbonne Paris Cité, Paris, France
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Paris, France - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
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    19.12.2018
Published in:
  • Journal of Clinical Microbiology. - 2018
English A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its evaluation was performed by using 92 colistin- resistant and colistin-susceptible Acinetobacter baumannii and Pseudomonas aeruginosa isolates. Sensitivity and specificity were respectively found to be 100 and 95% by comparison with the standard broth microdilution method. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is inexpensive, easy-to-perform, highly sensitive and specific, and can be completed in 4 hours. It could be useful in countries facing endemic spread of colistin-resistant non fermenters.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307791
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