A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde; Poirel, Laurent; Tinguely, Camille; Nordmann, Patrice

doi:10.1128/JCM.01563-18

Back

Journal article

A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - Université Paris Nord, Sorbonne Paris Cité, Paris, France - Université Paris Nord, Sorbonne Paris Cité, Paris, France
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Paris, France
Tinguely, Camille Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - Université Paris Nord, Sorbonne Paris Cité, Paris, France - Université Paris Nord, Sorbonne Paris Cité, Paris, France
Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris France - AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Paris, France - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland

19.12.2018

Published in:

Journal of Clinical Microbiology. - 2019, vol. 57, no. 3, p. 10.1128/jcm.01563-18

English A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its evaluation was performed by using 92 colistin- resistant and colistin-susceptible Acinetobacter baumannii and Pseudomonas aeruginosa isolates. Sensitivity and specificity were respectively found to be 100 and 95% by comparison with the standard broth microdilution method. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is inexpensive, easy-to-perform, highly sensitive and specific, and can be completed in 4 hours. It could be useful in countries facing endemic spread of colistin-resistant non fermenters.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language