Journal article

Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

  • Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
  • Kieffer, Nicolas Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
  • Guérind, François Service de Microbiologie, CHU de Caen, France
  • Güneser, Deniz Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland
  • Cattoir, Vincent Laboratoire de Bactériologie, CHU de Rennes, France
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland - University of Lausanne and University Hospital Center, Lausanne, Switzerland
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    01.10.2018
Published in:
  • Diagnostic Microbiology and Infectious Disease. - 2018, vol. 92, no. 2, p. 90–94
English The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin- susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3 hours of incubation. The results were compared with those of the homemade Rapid Polymyxin NP test, and manual broth microdilution according to EUCAST guidelines was used as the reference method to determine the performance of the test. Excellent performance of the commercial Rapid Polymyxin NP test was found with a very major error rate, a major error rate, a sensitivity, and a specificity of 1.9%, 5.1%, 98.1%, and 94.9%, respectively. The performance of the homemade Polymyxin NP test was similar, with a slightly better value for the very major error (1.2 %).
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307443
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