Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

Jayol, Aurélie; Kieffer, Nicolas; Poirel, Laurent; Guérind, François; Güneser, Deniz; Cattoir, Vincent; Nordmann, Patrice

doi:10.1016/j.diagmicrobio.2018.05.006

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Journal article

Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
Kieffer, Nicolas Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland
Guérind, François Service de Microbiologie, CHU de Caen, France
Güneser, Deniz Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland
Cattoir, Vincent Laboratoire de Bactériologie, CHU de Rennes, France
Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - French INSERM European Unit, University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland - University of Lausanne and University Hospital Center, Lausanne, Switzerland

01.10.2018

Published in:

Diagnostic Microbiology and Infectious Disease. - 2018, vol. 92, no. 2, p. 90–94

English The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin- susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3 hours of incubation. The results were compared with those of the homemade Rapid Polymyxin NP test, and manual broth microdilution according to EUCAST guidelines was used as the reference method to determine the performance of the test. Excellent performance of the commercial Rapid Polymyxin NP test was found with a very major error rate, a major error rate, a sensitivity, and a specificity of 1.9%, 5.1%, 98.1%, and 94.9%, respectively. The performance of the homemade Polymyxin NP test was similar, with a slightly better value for the very major error (1.2 %).

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language