Journal article

Integrated sensor system for DNA amplification and separation based on thin film technology

  • Costantini, Francesca School of Aerospace Engineering, Sapienza University of Rome, Italy
  • Petrucci, Giulia Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Italy
  • Lovecchio, Nicola Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Italy
  • Nardecchia, Marco Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Italy
  • Nascetti, Augusto School of Aerospace Engineering, Sapienza University of Rome, Italy
  • Cesare, Giampiero de Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Italy
  • Tedeschi, Lorena Institute of Clinical Physiology, Pisa, Italy
  • Domenici, Claudio Institute of Clinical Physiology, Pisa, Italy
  • Ruggi, Albert Department of Chemistry, University of Fribourg, Switzerland
  • Placidi, Pisana Department of Engineering, University of Perugia, Perugia, Italy
  • Scorzoni, Andrea Department of Engineering, University of Perugia, Perugia, Italy
  • Caputo, Domenico Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Italy
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    2018
Published in:
  • IEEE Transactions on Components, Packaging and Manufacturing Technology. - 2018, vol. 8, no. 7, p. 1141–1148
French This paper presents the development of a lab-on-chip, based on thin-film sensors, suitable for DNA treatments. In particular, the system performs on-chip DNA amplification and separation of double-strand DNA into single-strand DNA, combining a polydimethylsiloxane microfluidic network, thin-film electronic devices, and surface chemistry. Both the analytical procedures rely on the integration on the same glass substrate of thin-film metal heaters and amorphous silicon temperature sensors to achieve a uniform temperature distribution (within ±1 °C) in the heated area and a precise temperature control (within ±0.5 °C). The DNA separation also counts on the binding between biotinylated dsDNA and a layer of streptavidin immobilized into a microfluidic channel through polymer-brushes-based layer. This approach results in a fast and low reagents consumption system. The tested DNA treatments can be applied for carrying out the on-chip systematic evolution of ligands by exponential enrichment process, a chemistry technique for the selection of aptamers.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307262
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