Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients
- Zurfluh, Katrin Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Switzerland
- Stephan, Roger Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Switzerland
- Widmer, Andreas Division of Infectious Diseases and Hospital Epidemiology, University of Basel, Switzerland
- Poirel, Laurent Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
- Nordmann, Patrice Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
- Nüesch, Hans-Jakob Practice for General Medicine, Seuzach, Switzerland
- Hächler, Herbert Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Switzerland
- Nüesch-Inderbinen, Magdalena Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Switzerland
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15.12.2016
Published in:
- Antimicrobial Resistance & Infection Control. - 2017, vol. 6, p. 28
English
The objective of this study was to test the efficacy of an inhibitor of the New Delhi metallo-β- lactamase (NDM-1). Inhibiting expression of this type of antibiotic-resistance gene has the potential to restore antibiotic susceptibility in all bacteria carrying the gene.Methods: We have constructed a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) that selectively inhibits the expression of NDM-1 and examined its ability to restore susceptibility to meropenem in vitro and in vivo.Results:In vitro, the PPMO reduced the MIC of meropenem for three different genera of pathogens that express NDM-1. In a murine model of lethal E. coli sepsis, the PPMO improved survival (92%) and reduced systemic bacterial burden when given concomitantly with meropenem.Conclusions: These data show that a PPMO can restore antibiotic susceptibility in vitro and in vivo and that the combination of PPMO and meropenem may have therapeutic potential against certain class B carbapenem- resistant infections in multiple genera of Gram-negative pathogens.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 288699
- DOI 10.1186/s13756-017-0186-z
- Persistent URL
- https://folia.unifr.ch/unifr/documents/305517
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