TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics

Zhu, Jinsheng; Bailly, Aurelien; Zwiewka, Marta; Sovero, Valpuri; Donato, Martin Di; Ge, Pei; Oehri, Jacqueline; Aryal, Bibek; Hao, Pengchao; Linnert, Miriam; Burgardt, Noelia Inés; Lücke, Christian; Weiwad, Matthias; Michel, Max; Weiergräber, Oliver H.; Pollmann, Stephan; Azzarello, Elisa; Mancuso, Stefano; Ferro, Noel; Fukao, Yoichiro; Hoffmann, Céline; Wedlich-Söldner, Roland; Friml, Jiří; Thomas, Clément; Geisler, Markus

doi:10.1105/tpc.15.00726

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TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics

Zhu, Jinsheng Department of Biology, University of Fribourg, Switzerland - Structural Plant Biology Laboratory, Department of Botany and Plant Biology, Geneva, Switzerland
Bailly, Aurelien Department of Biology, University of Fribourg, Switzerland - Department of Plant and Microbial Biology, University of Zurich, Switzerland
Zwiewka, Marta CEITEC-Central European Institute of Technology, Masaryk University, Brno, Czech Republic
Sovero, Valpuri Department of Plant and Microbial Biology, University of Zurich, Switzerland
Donato, Martin Di Department of Biology, University of Fribourg, Switzerland
Ge, Pei Department of Biology, University of Fribourg, Switzerland - Station Biologique de Roscoff, CNRS-UPMC, France
Oehri, Jacqueline Department of Biology, University of Fribourg, Switzerland - Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland
Aryal, Bibek Department of Biology, University of Fribourg, Switzerland
Hao, Pengchao Department of Biology, University of Fribourg, Switzerland
Linnert, Miriam Max Planck Research Unit for Enzymology of Protein Folding, Halle (Saale), Germany - Faunhofer Institute for Cell Therapy and Immunology IZI, Department of Drug Design and Target Validation, Halle, Germany
Burgardt, Noelia Inés Max Planck Research Unit for Enzymology of Protein Folding, Halle (Saale), Germany - Institute of Biochemistry and Biophysics (IQUIFIB), School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina
Lücke, Christian Max Planck Research Unit for Enzymology of Protein Folding, Halle (Saale), Germany
Weiwad, Matthias Max Planck Research Unit for Enzymology of Protein Folding, Halle (Saale), Germany - Department of Enzymology, Martin-Luther-University Halle-Wittenberg, Institute of Biochemistry and Biotechnology, Halle, Germany
Michel, Max Institute of Complex Systems, ICS-6: Structural Biochemistry, Jülich, Germany
Weiergräber, Oliver H. Institute of Complex Systems, ICS-6: Structural Biochemistry, Jülich, Germany
Pollmann, Stephan Centro de Biotecnología y Genómica de Plantas, Pozuelo de Alarcón, Madrid, Spain
Azzarello, Elisa LINV-DIPSAA, Università di Firenze, Italy
Mancuso, Stefano LINV-DIPSAA, Università di Firenze, Italy
Ferro, Noel University of Bonn, Mulliken Center for Theoretical Chemistry, Institute for Physical and Theoretical Chemistry, Bonn, Germany
Fukao, Yoichiro Plant Global Educational Project, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan - Department of Bioinfomatics, Ritsumeikan University, Shiga, Japan
Hoffmann, Céline Cytoskeleton and Cancer Progression, Laboratory of Experimental Cancer Research, Department of Oncology, Luxembourg Institute of Health, Luxembourg
Wedlich-Söldner, Roland Institute of Cell Dynamics and Imaging, University of Münster, Germany
Friml, Jiří Institute of Science and Technology, Klosterneuburg, Austria
Thomas, Clément Cytoskeleton and Cancer Progression, Laboratory of Experimental Cancer Research, Department of Oncology, Luxembourg Institute of Health, Luxembourg
Geisler, Markus Department of Biology, University of Fribourg, Switzerland - Department of Plant and Microbial Biology, University of Zurich, Switzerland

01.04.2016

Published in:

The Plant Cell. - 2016, vol. 28, no. 4, p. 930–948

English Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis thaliana ABCB chaperone TWISTED DWARF1 (TWD1). We identify ACTIN7 as a relevant, although likely indirect, TWD1 interactor, and show TWD1-dependent regulation of actin filament organization and dynamics and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters, and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide evidence that TWD1 determines downstream locations of auxin efflux transporters by adjusting actin filament debundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

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