Journal article

Rapid detection of polymyxin-resistant Enterobacteriaceae from blood cultures

  • Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, France), University of Fribourg, Switzerland - CNRS UMR5234, University of Bordeaux, France
  • Dubois, Véronique CNRS UMR5234, University of Bordeaux, France
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, France), University of Fribourg, Switzerland
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    15.06.2016
Published in:
  • Journal of Clinical Microbiology. - 2016, p. JCM.00918-16
English Enterobacterial strains resistant to polymyxins are increasingly reported worldwide. Conventional methods for detection of colistin-resistant isolates such as broth microdilution remain time-consuming (24 to 48 h), and methods such as disc diffusion and E-test are not reliable. Recently, the Rapid Polymyxin NP test was developed for a rapid identification of polymyxin-resistant Enterobacteriaceae. This test is based on the detection of glucose metabolism related to bacterial growth in the presence of a defined concentration of colistin (or polymyxin B). Formation of acid metabolites is evidenced by a color change of a pH indicator (red phenol) in less than 2h. In this study, the Polymyxin NP test has been evaluated to detect colistin-resistant Enterobacteriaceae directly from blood cultures. The test was performed with 73 blood culture sets (either spiked or clinical blood cultures) with various enterobacterial species. The test exhibited excellent discrimination between polymyxin-resistant and polymyxin- susceptible enterobacterial isolates, and results are obtained from blood cultures within 4 hours. It is easy to perform, and neither requires subculture nor centrifugation step. This test is rapid, specific, and sensitive, and allows early identification of polymyxin- resistant Enterobacteriaceae directly from blood cultures.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304865
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