Journal article
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The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis
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Numrich, Johannes
University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
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Péli-Gulli, Marie-Pierre
University of Fribourg, Department of Biology, Division of Biochemistry, Switzerland
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Arlt, Henning
University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
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Sardu, Alessandro
University of Fribourg, Department of Biology, Division of Biochemistry, Switzerland
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Griffith, Janice
University Medical Centre Utrecht, Center for Molecular Medicine, Department of Cell Biology, The Netherlands
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Levine, Tim
UCL Institute of Ophthalmology, Department of Cell Biology, London, UK
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Engelbrecht-Vandré, Siegfried
University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
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Reggiori, Fulvio
University Medical Centre Utrecht, Center for Molecular Medicine, Department of Cell Biology, The Netherlands
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De Virgilio, Claudio
University of Fribourg, Department of Biology, Division of Biochemistry, Switzerland
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Ungermann, Christian
University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
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Published in:
- Journal of Cell Science. - 2015, vol. 128, no. 13, p. 2278–2292
English
Membrane fusion at the vacuole depends on a conserved machinery that includes SNAREs, the Rab7 homolog Ypt7 and its effector HOPS. Here, we demonstrate that Ypt7 has an unexpected additional function by controlling membrane homeostasis and nutrient-dependent signaling on the vacuole surface. We show that Ivy1, the yeast homolog of mammalian missing-in-metastasis (MIM), is a vacuolar effector of Ypt7-GTP and interacts with the EGO/ragulator complex, an activator of the target of rapamycin kinase complex 1 (TORC1) on vacuoles. Loss of Ivy1 does not affect EGO vacuolar localization and function. In combination with the deletion of individual subunits of the V-ATPase, however, we observed reduced TORC1 activity and massive enlargement of the vacuole surface. Consistent with this, Ivy1 localizes to invaginations at the vacuole surface and on liposomes in a phosphoinositide- and Ypt7-GTP-controlled manner, which suggests a role in microautophagy. Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Biologie
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/304371
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