Journal article

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The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis

  • Numrich, Johannes University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
  • Péli-Gulli, Marie-Pierre University of Fribourg, Department of Biology, Division of Biochemistry, Switzerland
  • Arlt, Henning University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
  • Sardu, Alessandro University of Fribourg, Department of Biology, Division of Biochemistry, Switzerland
  • Griffith, Janice University Medical Centre Utrecht, Center for Molecular Medicine, Department of Cell Biology, The Netherlands
  • Levine, Tim UCL Institute of Ophthalmology, Department of Cell Biology, London, UK
  • Engelbrecht-Vandré, Siegfried University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
  • Reggiori, Fulvio University Medical Centre Utrecht, Center for Molecular Medicine, Department of Cell Biology, The Netherlands
  • De Virgilio, Claudio University of Fribourg, Department of Biology, Division of Biochemistry, Switzerland
  • Ungermann, Christian University of Osnabrück, Department of Biology/Chemistry, Biochemistry section, Germany
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    07.01.2015
Published in:
  • Journal of Cell Science. - 2015, vol. 128, no. 13, p. 2278–2292
English Membrane fusion at the vacuole depends on a conserved machinery that includes SNAREs, the Rab7 homolog Ypt7 and its effector HOPS. Here, we demonstrate that Ypt7 has an unexpected additional function by controlling membrane homeostasis and nutrient-dependent signaling on the vacuole surface. We show that Ivy1, the yeast homolog of mammalian missing-in-metastasis (MIM), is a vacuolar effector of Ypt7-GTP and interacts with the EGO/ragulator complex, an activator of the target of rapamycin kinase complex 1 (TORC1) on vacuoles. Loss of Ivy1 does not affect EGO vacuolar localization and function. In combination with the deletion of individual subunits of the V-ATPase, however, we observed reduced TORC1 activity and massive enlargement of the vacuole surface. Consistent with this, Ivy1 localizes to invaginations at the vacuole surface and on liposomes in a phosphoinositide- and Ypt7-GTP-controlled manner, which suggests a role in microautophagy. Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304371
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