OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa.L)
- Xu, YanXia State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
- Zhang, SaiNa State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
- Guo, HaiPeng State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
- Wang, SuiKang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
- Xu, LiGen State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
- Li, ChuanYou State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
- Qian, Qian State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
- Chen, Fan State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
- Geisler, Markus Department of Biology - Plant Biology, University of Fribourg, Switzerland
- Qi, YanHua State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
- Jiang, DeAn State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
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01.05.2014
Published in:
- The Plant Journal. - 2014, p. –
English
Members of the ATP Binding Cassette B/Multidrug-resistance/P-glycoprotein (ABCB/MDR/PGP) subfamily were shown to function primarily in auxin transport. However, none of rice ABCB transporters have been functionally characterized. Here, we describe that a knock-down of OsABCB14 confers decreased auxin concentrations and polar auxin transport rates, conferring insensitivity to 2,4-D and IAA. OsABCB14 displays enhanced specific auxin influx activity in yeast and protoplasts prepared from rice knock-down alleles. OsABCB14 is localized at the plasma membrane pointing to an important directionality under physiological conditions. osabcb14 mutants were surprisingly found to be insensitive to iron deficiency treatment (–Fe). Their Fe concentration is higher and up-regulation of Fe-deficiency responsive genes is lower in osabcb14 mutants than in wild type rice, Nipponbare (NIP). Taken together, our results strongly support the role of OsABCB14 as an auxin influx transporter involved in iron homeostasis. The functional characterization of OsABCB14 provides insights in monocot auxin transport and its relationship to Fe nutrition.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 210213
- DOI 10.1111/tpj.12544
- Persistent URL
- https://folia.unifr.ch/unifr/documents/303813
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