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A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes

  • Clift, Martin J. D. Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
  • Endes, Carola Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
  • Vanhecke, Dimitri Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
  • Wick, Peter Empa, Swiss Federal Laboratories for Material Science and Technology, Materials-Biology Interactions Laboratory, St. Gallen, Switzerland
  • Gehr, Peter Institute of Anatomy, University of Bern, Switzerland
  • Schins, Roel P. F. Institute for Umweltmedizinische Forschung (IUF), Heinrich-Heine-Universitat Düsseldorf, Germany
  • Petri-Fink, Alke Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
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    01.01.2014
Published in:
  • Toxicological Sciences. - 2014, vol. 137, no. 1, p. 55-64
In vitro lung systems
Carbon nanotubes
Nanotoxicology
Oxidative stress
Inflammation
Risk assessment
English To determine the potential inhalatory risk posed by carbon nanotubes (CNTs), a tier-based approach beginning with an in vitro assessment must be adopted. The purpose of this study therefore was to compare 4 commonly used in vitro systems of the human lung (human blood monocyte-derived macrophages [MDM] and monocyte-derived dendritic cells [MDDC], 16HBE14o- epithelial cells, and a sophisticated triple cell co-culture model [TCC-C]) via assessment of the biological impact of different CNTs (single-walled CNTs [SWCNTs] and multiwalled CNTs [MWCNTs]) over 24h. No significant cytotoxicity was observed with any of the cell types tested, although a significant (p < .05), dose-dependent increase in tumor necrosis factor (TNF)-α following SWCNT and MWCNT exposure at concentrations up to 0.02mg/ml to MDM, MDDC, and the TCC-C was found. The concentration of TNF-α released by the MDM and MDDC was significantly higher (p < .05) than the TCC-C. Significant increases (p < .05) in interleukin (IL)-8 were also found for both 16HBE14o- epithelial cells and the TCC-C after SWCNTs and MWCNTs exposure up to 0.02mg/ml. The TCC-C, however, elicited a significantly (p < .05) higher IL-8 release than the epithelial cells. The oxidative potential of both SWCNTs and MWCNTs (0.005–0.02mg/ml) measured by reduced glutathione (GSH) content showed a significant difference (p < .05) between each monoculture and the TCC-C. It was concluded that because only the co-culture system could assess each endpoint adequately, that, in comparison with monoculture systems, multicellular systems that take into consideration important cell type-to-cell type interactions could be used as predictive in vitro screening tools for determining the potential deleterious effects associated with CNTs.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/303476
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