Journal article

A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes

  • Clift, Martin J. D. Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
  • Endes, Carola Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
  • Vanhecke, Dimitri Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
  • Wick, Peter Empa, Swiss Federal Laboratories for Material Science and Technology, Materials-Biology Interactions Laboratory, St. Gallen, Switzerland
  • Gehr, Peter Institute of Anatomy, University of Bern, Switzerland
  • Schins, Roel P. F. Institute for Umweltmedizinische Forschung (IUF), Heinrich-Heine-Universitat Düsseldorf, Germany
  • Petri-Fink, Alke Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Bio-Nanomaterials, Adolphe Merkle Institute, Fribourg, Switzerland
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Published in:
  • Toxicological Sciences. - 2014, vol. 137, no. 1, p. 55-64
English To determine the potential inhalatory risk posed by carbon nanotubes (CNTs), a tier-based approach beginning with an in vitro assessment must be adopted. The purpose of this study therefore was to compare 4 commonly used in vitro systems of the human lung (human blood monocyte-derived macrophages [MDM] and monocyte-derived dendritic cells [MDDC], 16HBE14o- epithelial cells, and a sophisticated triple cell co-culture model [TCC-C]) via assessment of the biological impact of different CNTs (single-walled CNTs [SWCNTs] and multiwalled CNTs [MWCNTs]) over 24h. No significant cytotoxicity was observed with any of the cell types tested, although a significant (p < .05), dose-dependent increase in tumor necrosis factor (TNF)-α following SWCNT and MWCNT exposure at concentrations up to 0.02mg/ml to MDM, MDDC, and the TCC-C was found. The concentration of TNF-α released by the MDM and MDDC was significantly higher (p < .05) than the TCC-C. Significant increases (p < .05) in interleukin (IL)-8 were also found for both 16HBE14o- epithelial cells and the TCC-C after SWCNTs and MWCNTs exposure up to 0.02mg/ml. The TCC-C, however, elicited a significantly (p < .05) higher IL-8 release than the epithelial cells. The oxidative potential of both SWCNTs and MWCNTs (0.005–0.02mg/ml) measured by reduced glutathione (GSH) content showed a significant difference (p < .05) between each monoculture and the TCC-C. It was concluded that because only the co-culture system could assess each endpoint adequately, that, in comparison with monoculture systems, multicellular systems that take into consideration important cell type-to-cell type interactions could be used as predictive in vitro screening tools for determining the potential deleterious effects associated with CNTs.
Faculté des sciences et de médecine
Département de Chimie
  • English
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