Strategy for a rapid detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent; Bréchard, Ludivine; Cuzon, Gaëlle; Poirel, Laurent; Nordmann, Patrice

doi:10.1128/AAC.01239-13

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Journal article

Strategy for a rapid detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent INSERM U914, South-Paris Medical School, K.-Bicêtre, France
Bréchard, Ludivine INSERM U914, South-Paris Medical School, K.-Bicêtre, France
Cuzon, Gaëlle INSERM U914, South-Paris Medical School, K.-Bicêtre, France
Poirel, Laurent INSERM U914, South-Paris Medical School, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
Nordmann, Patrice INSERM U914, South-Paris Medical School, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland

27.01.2014

Published in:

Antimicrobial Agents and Chemotherapy. - 2014, vol. 58, no. 4, p. 2441-2445

English A prospective survey was conducted on 862 Enterobacteriaceae with reduced susceptibility to carbapenems. The Carba NP test, UV spectrophotometry and DNA microarray were used for detecting carbapenemase producers, and results compared to PCR and sequencing. The 172 carbapenemase producers were detected using the Carba NP test and UV spectrophotometry whereas DNA microarray failed to detect IMI producers. Use of the Carba NP test as first screening, followed by the use of molecular techniques has been defined as an efficient strategy for identification of carbapenemase-producing Enterobacteriaceae.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language