Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs

Guex, Anne Géraldine; Birrer, Dominique Lisa; Fortunato, Giuseppino; Tevaearai, Hendrik Tinorua; Giraud, Marie-Noëlle

doi:10.1088/1748-6041/8/2/021001

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Journal article

Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs

Guex, Anne Géraldine Empa—Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland - Clinic for Cardiovascular Surgery, Inselspital Bern, University Hospital and University of Bern, Switzerland
Birrer, Dominique Lisa Clinic for Cardiovascular Surgery, Inselspital Bern, University Hospital and University of Bern, Switzerland
Fortunato, Giuseppino Empa—Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland -
Tevaearai, Hendrik Tinorua Clinic for Cardiovascular Surgery, Inselspital Bern, University Hospital and University of Bern, Switzerland
Giraud, Marie-Noëlle Cardiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland

01.04.2013

Published in:

Biomedical Materials. - 2013, vol. 8, no. 2, p. 021001

English Engineered muscle constructs provide a promising perspective on the regeneration or substitution of irreversibly damaged skeletal muscle. However, the highly ordered structure of native muscle tissue necessitates special consideration during scaffold development. Multiple approaches to the design of anisotropically structured substrates with grooved micropatterns or parallel-aligned fibres have previously been undertaken. In this study we report the guidance effect of a scaffold that combines both approaches, oriented fibres and a grooved topography. By electrospinning onto a topographically structured collector, matrices of parallel-oriented poly(ε-caprolactone) fibres with an imprinted wavy topography of 90 µm periodicity were produced. Matrices of randomly oriented fibres or parallel-oriented fibres without micropatterns served as controls. As previously shown, un-patterned, parallel-oriented substrates induced myotube orientation that is parallel to fibre direction. Interestingly, pattern addition induced an orientation of myotubes at an angle of 24° (statistical median) relative to fibre orientation. Myotube length was significantly increased on aligned micropatterned substrates in comparison to that on aligned substrates without pattern (436 ± 245 µm versus 365 ± 212 µm; p < 0.05). We report an innovative, yet simple, design to produce micropatterned electrospun scaffolds that induce an unexpected myotube orientation and an increase in myotube length.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language