Journal article
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Yeast endosulfines control entry into quiescence and chronological life span by inhibiting protein phosphatase 2A
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Bontron, Séverine
Department of Biology, Division of Biochemistry, University of Fribourg, Switzerland
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Jaquenoud, Malika
Department of Biology, Division of Biochemistry, University of Fribourg, Switzerland
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Vaga, Stefania
Institute of Molecular Systems Biology, ETH Zürich, Switzerland
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Talarek, Nicolas
Department of Biology, Division of Biochemistry, University of Fribourg, Switzerland
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Bodenmiller, Bernd
Institute of Molecular Systems Biology, ETH Zürich, Switzerland
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Aebersold, Ruedi
Institute of Molecular Systems Biology, ETH Zürich, Switzerland
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De Virgilio, Claudio
Department of Biology, Division of Biochemistry, University of Fribourg, Switzerland
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Published in:
- Cell Reports. - 2012, vol. 3, no. 1, p. 16–22
English
The TORC1 and PKA protein kinases are central elements of signaling networks that regulate eukaryotic cell proliferation in response to growth factors and/or nutrients. In yeast, attenuation of signaling by these kinases following nitrogen and/or carbon limitation activates the protein kinase Rim15, which orchestrates the initiation of a reversible cellular quiescence program to ensure normal chronological life span. The molecular elements linking Rim15 to distal readouts including the expression of Msn2/4- and Gis1-dependent genes involve the endosulfines Igo1/2. Here, we show that Rim15, analogous to the greatwall kinase in Xenopus, phosphorylates endosulfines to directly inhibit the Cdc55-protein phosphatase 2A (PP2ACdc55). Inhibition of PP2ACdc55 preserves Gis1 in a phosphorylated state and consequently promotes its recruitment to and activation of transcription from promoters of specific nutrient-regulated genes. These results close a gap in our perception of and delineate a role for PP2ACdc55 in TORC1-/PKA-mediated regulation of quiescence and chronological life span.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Biologie
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/302730
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