Journal article

Regulation of ABCB1/PGP1-catalysed auxin transport by linker phosphorylation

  • Henrichs, Sina Molecular Plant Physiology, Institute of Plant Biology, University of Zurich and Zurich-Basel Plant Science Center, Zurich, Switzerland -
  • Wang, Bangjun Molecular Plant Physiology, Institute of Plant Biology, University of Zurich and Zurich-Basel Plant Science Center, Zurich, Switzerland - Department of Biology—Plant Biology, University of Fribourg, Switzerland - Department of Plant Biology and Biotechnology, University of Copenhagen, Frederiksberg, Denmark -
  • Fukao, Yoichiro Plant Global Educational Project, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan
  • Zhu, Jinsheng Department of Biology—Plant Biology, University of Fribourg, Switzerland
  • Charrier, Laurence Department of Biology—Plant Biology, University of Fribourg, Switzerland
  • Bailly, Aurélien Molecular Plant Physiology, Institute of Plant Biology, University of Zurich and Zurich-Basel Plant Science Center, Zurich, Switzerland - Department of Biology—Plant Biology, University of Fribourg, Switzerland
  • Oehring, Sophie C Molecular Plant Physiology, Institute of Plant Biology, University of Zurich and Zurich-Basel Plant Science Center, Zurich, Switzerland -
  • Linnert, Miriam Signaltransduktion, Max-Planck-Forschungsstelle für Enzymologie der Proteinfaltung, Halle (Saale), Germany
  • Weiwad, Matthias Signaltransduktion, Max-Planck-Forschungsstelle für Enzymologie der Proteinfaltung, Halle (Saale), Germany
  • Endler, Anne Molecular Plant Physiology, Institute of Plant Biology, University of Zurich and Zurich-Basel Plant Science Center, Zurich, Switzerland -
  • Nanni, Paolo Functional Genomics Center Zurich, UZH/ETH Zürich, Zürich, Switzerland
  • Pollmann, Stephan Ruhr-Universität Bochum, Lehrstuhl für Pflanzenphysiologie, Germany -
  • Mancuso, Stefano Department of Plant, Soil and Environmental Science, University of Florence, Sesto Fiorentino, Italy
  • Schulz, Alexander Department of Plant Biology and Biotechnology, University of Copenhagen, Frederiksberg, Denmark
  • Geisler, Markus Molecular Plant Physiology, Institute of Plant Biology, University of Zurich and Zurich-Basel Plant Science Center, Zurich, Switzerland - Department of Biology—Plant Biology, University of Fribourg, Switzerland
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    01.05.2012
Published in:
  • The EMBO Journal. - 2012, vol. 31, no. 13, p. 2965-2980
English Polar transport of the plant hormone auxin is controlled by PIN- and ABCB/PGP-efflux catalysts. PIN polarity is regulated by the AGC protein kinase, PINOID (PID), while ABCB activity was shown to be dependent on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Using co-immunoprecipitation (co-IP) and shotgun LC–MS/MS analysis, we identified PID as a valid partner in the interaction with TWD1. In-vitro and yeast expression analyses indicated that PID specifically modulates ABCB1-mediated auxin efflux in an action that is dependent on its kinase activity and that is reverted by quercetin binding and thus inhibition of PID autophosphorylation. Triple ABCB1/PID/TWD1 co-transfection in tobacco revealed that PID enhances ABCB1-mediated auxin efflux but blocks ABCB1 in the presence of TWD1. Phospho-proteomic analyses identified S634 as a key residue of the regulatory ABCB1 linker and a very likely target of PID phosphorylation that determines both transporter drug binding and activity. In summary, we provide evidence that PID phosphorylation has a dual, counter-active impact on ABCB1 activity that is coordinated by TWD1–PID interaction.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302535
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