Journal article

Measuring multiple neurochemicals and related metabolites in blood and brain of the rhesus monkey by using dual microdialysis sampling and capillary hydrophilic interaction chromatography–mass spectrometry

  • Li, Juan Max Planck Institute for Biological Cybernetics, Tübingen, Germany
  • Pföstl, Veronika von Max Planck Institute for Biological Cybernetics, Tübingen, Germany
  • Zaldivar, Daniel Max Planck Institute for Biological Cybernetics, Tübingen, Germany - Graduate School of Neural and Behavioural Science/International Max Planck Research School, University Tübingen, Germany
  • Zhang, Xiaozhe Department of Medicine, University of Fribourg, Switzerland
  • Logothetis, Nikos Max Planck Institute for Biological Cybernetics, Tübingen, Germany - Division of Imaging Science and Biomedical Engineering, University of Manchester, UK
  • Rauch, Alexander Max Planck Institute for Biological Cybernetics, Tübingen, Germany
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  • Analytical and Bioanalytical Chemistry. - 2012, vol. 402, no. 8, p. 2545-2554
English In vivo measurement of multiple functionally related neurochemicals and metabolites (NMs) is highly interesting but remains challenging in the field of basic neuroscience and clinical research. We present here an analytical method for determining five functionally and metabolically related polar substances, including acetylcholine (quaternary ammonium), lactate and pyruvate (organic acids), as well as glutamine and glutamate (amino acids). These NMs are acquired from samples of the brain and the blood of non-human primates in parallel by dual microdialysis, and subsequently analyzed by a direct capillary hydrophilic interaction chromatography (HILIC)–mass spectrometry (MS) based method. To obtain high sensitivity in electrospray ionization (ESI)–MS, lactate and pyruvate were detected in negative ionization mode whereas the other NMs were detected in positive ionization mode during each HILIC-MS run. The method was validated for linearity, the limits of detection and quantification, precision, accuracy, stability and matrix effect. The detection limit of acetylcholine, lactate, pyruvate, glutamine, and glutamate was 150 pM, 3 µM, 2 µM, 5 nM, and 50 nM, respectively. This allowed us to quantitatively and simultaneously measure the concentrations of all the substances from the acquired dialysates. The concentration ratios of both lactate/pyruvate and glutamine/glutamate were found to be higher in the brain compared to blood (p <0.05). The reliable and simultaneous quantification of these five NMs from brain and blood samples allows us to investigate their relative distribution in the brain and blood, and most importantly paves the way for future non-invasive studies of the functional and metabolic relation of these substances to each other.
Faculté des sciences et de médecine
Département de Médecine
  • English
Biological sciences
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