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Quantification of purine basis in their mixtures at femto-molar concentration levels using FT-SERS

  • Ranc, Vaclav Department of Physiology, Faculty of Medicine, University of Fribourg, Switzerland
  • Hruzikova, Jana Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
  • Maitner, Kamil Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
  • Prucek, Robert Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
  • Milde, David Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
  • Kvítek, Libor Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
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    22.12.2011
Published in:
  • Journal of Raman Spectroscopy. - 2012, vol. 43, no. 8, p. 971-976
FT-SERS
adenine
hypoxanthine
xanthine
Raman spectrometry
nucleotides
nanoparticle
English Surface-enhanced Raman scattering spectroscopy represents one of the unique techniques for studying nanoscale objects, and its distinctive properties can be used in the process of further analysis. The careful evaluation of the particular influence of selected key-role experimental parameters (e.g. pH value of measured sample mixture, size and distribution of used nanoparticles) and the influence of reduction agent used in the process of formation of desired nanoparticle objects presents an important task in the further study of surface-enhanced Raman scattering effect. A broad study of these experimental parameters was performed in this paper. The main aim of the presented work was to a demonstrate an application potential of selected experimental conditions in the determination of three purine bases: adenine, xanthine, and hypoxanthine. The resulting limits of detection are at femtomolar concentration levels for all three studied compounds.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302275
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