Over-expression of a protein kinase gene enhances the defense of tobacco against Rhizoctonia solani
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Chacón, Osmany
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba - Tobacco Research Institute, San Antonio de los Baños, Havana, Cuba
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González, Marleny
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba - Plant Health Institute, Playa, Havana, Cuba
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López, Yunior
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
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Portieles, Roxana
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
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Pujol, Merardo
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
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González, Ernesto
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
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Schoonbeek, Henk-jan
Department of Biology, University of Fribourg, Switzerland
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Métraux, Jean-Pierre
Department of Biology, University of Fribourg, Switzerland
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Borrás-Hidalgo, Orlando
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
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Published in:
- Gene. - 2010, vol. 45, no. 2, p. 54-62
English
To identify Nicotiana tabacum genes involved in resistance and susceptibility to Rhizoctonia solani, suppression subtractive hybridization was used to generate a cDNA library from transcripts that are differentially expressed during a compatible and incompatible interaction. This allowed the isolation of a protein kinase cDNA that was down-regulated during a compatible and up-regulated during an incompatible interaction. Quantitative RT–PCR analysis of this gene confirmed the differential expression patterns between the compatible and incompatible interactions. Over-expression of this gene in tobacco enhanced the resistance to damping-off produced by an aggressive R. solani strain. Furthermore, silencing of this protein kinase gene reduced the resistance to a non-aggressive R. solani strain. A set of reported tobacco-resistant genes were also evaluated in tobacco plants over-expressing and silencing the protein kinase cDNA. Several genes previously associated with resistance in tobacco, like manganese superoxide dismutase, Hsr203J, chitinases and phenylalanine ammonia-lyase, were up-regulated in tobacco plants over-expressing the protein kinase cDNA. Potentially, the protein kinase gene could be used to engineer resistance to R. solani in tobacco cultivars susceptible to this important pathogen.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Biologie
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/301501
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