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Over-expression of a protein kinase gene enhances the defense of tobacco against Rhizoctonia solani

  • Chacón, Osmany Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba - Tobacco Research Institute, San Antonio de los Baños, Havana, Cuba
  • González, Marleny Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba - Plant Health Institute, Playa, Havana, Cuba
  • López, Yunior Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
  • Portieles, Roxana Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
  • Pujol, Merardo Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
  • González, Ernesto Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
  • Schoonbeek, Henk-jan Department of Biology, University of Fribourg, Switzerland
  • Métraux, Jean-Pierre Department of Biology, University of Fribourg, Switzerland
  • Borrás-Hidalgo, Orlando Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, Cuba
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    11.12.2009
Published in:
  • Gene. - 2010, vol. 45, no. 2, p. 54-62
Protein kinase
Rhizoctonia solani
Suppression subtractive hybridization
English To identify Nicotiana tabacum genes involved in resistance and susceptibility to Rhizoctonia solani, suppression subtractive hybridization was used to generate a cDNA library from transcripts that are differentially expressed during a compatible and incompatible interaction. This allowed the isolation of a protein kinase cDNA that was down-regulated during a compatible and up-regulated during an incompatible interaction. Quantitative RT–PCR analysis of this gene confirmed the differential expression patterns between the compatible and incompatible interactions. Over-expression of this gene in tobacco enhanced the resistance to damping-off produced by an aggressive R. solani strain. Furthermore, silencing of this protein kinase gene reduced the resistance to a non-aggressive R. solani strain. A set of reported tobacco-resistant genes were also evaluated in tobacco plants over-expressing and silencing the protein kinase cDNA. Several genes previously associated with resistance in tobacco, like manganese superoxide dismutase, Hsr203J, chitinases and phenylalanine ammonia-lyase, were up-regulated in tobacco plants over-expressing the protein kinase cDNA. Potentially, the protein kinase gene could be used to engineer resistance to R. solani in tobacco cultivars susceptible to this important pathogen.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/301501
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