The ABC transporter BcatrB from Botrytis cinerea exports camalexin and is a virulence factor on Arabidopsis thaliana
- Stefanato, Francesca L. Department of Biology, University of Fribourg, Switzerland
- Abou-Mansour, Eliane Department of Biology, University of Fribourg, Switzerland
- Buchala, Antony J. Department of Biology, University of Fribourg, Switzerland
- Kretschmer, Matthias Fachbereich Biologie, Technische Universität Kaiserslautern, Germany
- Mosbach, Andreas Fachbereich Biologie, Technische Universität Kaiserslautern, Germany
- Hahn, Matthias Fachbereich Biologie, Technische Universität Kaiserslautern, Germany
- Bochet, Christian G. Department of Chemistry, University of Fribourg, Switzerland
- Métraux, Jean-Pierre Department of Biology, University of Fribourg, Switzerland
- Schoonbeek, Henk-jan Department of Biology, University of Fribourg, Switzerland
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18.01.2009
Published in:
- The Plant Journal. - 2009, vol. 58, no. 3, p. 499-510
English
Arabidopsis thaliana is known to produce the phytoalexin camalexin in response to abiotic and biotic stress. Here we studied the mechanisms of tolerance to camalexin in the fungus Botrytis cinerea, a necrotrophic pathogen of A. thaliana. Exposure of B. cinerea to camalexin induces expression of BcatrB, an ABC transporter that functions in the efflux of fungitoxic compounds. B. cinerea inoculated on wild-type A. thaliana plants yields smaller lesions than on camalexin-deficient A. thaliana mutants. A B. cinerea strain lacking functional BcatrB is more sensitive to camalexin in vitro and less virulent on wild-type plants, but is still fully virulent on camalexin-deficient mutants. Pre-treatment of A. thaliana with UV-C leads to increased camalexin accumulation and substantial resistance to B. cinerea. UV-C-induced resistance was not seen in the camalexin-deficient mutants cyp79B2/B3, cyp71A13, pad3 or pad2, and was strongly reduced in ups1. Here we demonstrate that an ABC transporter is a virulence factor that increases tolerance of the pathogen towards a phytoalexin, and the complete restoration of virulence on host plants lacking this phytoalexin.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie, Département de Chimie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 11670
- DOI 10.1111/j.1365-313X.2009.03794.x
- Persistent URL
- https://folia.unifr.ch/unifr/documents/301166
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