The transillumination possibility of imidazole-osmium postfixed muscle tissue and its consequences for the handling of muscle tissue samples
- Voigt, Tilman Institute of Anatomy, University of Fribourg, Switzerland
- Dauber, Wolfgang Institute of Anatomy, Eberhard-Karls-University Tuebingen, Germany
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06.01.2004
Published in:
- Microscopy Research and Technique. - 2004, vol. 63(2), no. 129
English
The osmium postfixation of tissue leads to good results for transmission electron microscopy, but also produces completely blackened tissue samples that do not allow the recognition of internal structures. With imidazole-osmium postfixation, one achieves comparable results in high electron microscopic resolution as with routine osmium postfixation. But the tissue samples are not blackened and can, therefore, be transilluminated with point light sources. The new postfixation technique makes it possible to recognize histological details such as vessels, nerve fibers, and the cross-banding pattern in an untrimmed block. This makes it possible to screen-embedded tissue samples for appropriate ultrastructural processing.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Médecine
- Language
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- English
- Classification
- Biology
- License
- License undefined
- Identifiers
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- RERO DOC 5157
- DOI 10.1002/jemt.20021
- Persistent URL
- https://folia.unifr.ch/unifr/documents/299963
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