Preprint

Metagenome quality metrics and taxonomical annotation visualization through the integration of MAGFlow and BIgMAG

DOKPE

  • 2024
Submitted to:
  • F1000Research. - UK : Taylor and Francis Group / F1000Research. - 2024, vol. 13, p. 1-13
English Background: Building Metagenome–Assembled Genomes (MAGs) from highly complex metagenomics datasets encompasses a series of steps covering from cleaning the sequences, assembling them to finally group them into bins. Along the process, multiple tools aimed to assess the quality and integrity of each MAG are implemented. Nonetheless, even when incorporated within end–to–end pipelines, the outputs of these pieces of software must be visualized and analyzed manually lacking integration in a complete framework.

Methods: We developed a Nextflow pipeline (MAGFlow) for estimating the quality of MAGs through a wide variety of approaches (BUSCO, CheckM2, GUNC and QUAST), as well as for annotating taxonomically the metagenomes using GTDB-Tk2. MAGFlow is coupled to a Python–Dash application (BIgMAG) that displays the concatenated outcomes from the tools included by MAGFlow, highlighting the most important metrics in a single interactive environment along with a comparison/clustering of the input data.

Results: By using MAGFlow/BIgMAG, the user will be able to benchmark the MAGs obtained through different workflows or establish the quality of the MAGs belonging to different samples following the divide and rule methodology.

Conclusions: MAGFlow/BIgMAG represents a unique tool that integrates state-of- the-art tools to study different quality metrics and extract visually as much information as possible from a wide range of genome features.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biotechnology
License
CC BY
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/328840
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