Layer-by-Layer siRNA Particle Assemblies for Localized Delivery of siRNA to Epithelial Cells through Surface-Mediated Particle Uptake
DOKPE
- Lee, Aaron Adolphe Merkle Institute, University of Fribourg
- Gosnell, Natalia Adolphe Merkle Institute, University of Fribourg
- Milinkovic, Daela Adolphe Merkle Institute, University of Fribourg
- Taladriz-Blanco, Patricia International Iberian Nanotechnology Laboratory, Braga, Portugal
- Rothen-Rutishauser, Barbara ORCID Adolphe Merkle Institute, University of Fribourg
- Petri-Fink, Alke ORCID Adolphe Merkle Institute, University of Fribourg
- 2023
Published in:
- ACS Applied Bio Materials. - Washington, DC : American Chemical Society (ACS). - 2023, vol. 6, no. 1, p. 83-92 [1-31]
siRNA particles
localized delivery
epithelial cells
particle surface
layer-by-layer
particle uptake
drug delivery
transfection
English
Localized delivery of small interfering RNA (siRNA) is a promising approach to spatial control of cell responses at biomaterial interfaces. Layer-by-layer (LbL) assembly of siRNA with cationic polyelectrolytes has been used in film and nanoparticle vectors for transfection. Herein, we combine the ability of particles to efficiently deliver siRNA with the ability of film polyelectrolyte multilayers to act locally. LbL particles were prepared with alternating layers of poly(L-arginine) and siRNA and capped with hyaluronic acid. Negatively-charged LbL particles were subsequently assembled on poly(L-lysine)-functionalized substrate to form a LbL particle-decorated surface. Cells grown in contact with the particle-decorated surface were able to survive, internalize particles and undergo gene silencing. This work shows that particle-decorated surfaces can be engineered by using electrostatic interactions and used to deliver therapeutic payloads for cell-instructive biointerfaces.
- Faculty
- Faculté des sciences et de médecine
- Department
- NCCR - Bio-Inspired Materials
- Language
-
- English
- Classification
- Biological sciences
- License
- CC BY-NC-ND
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1021/acsabm.2c00668
- ISSN 2576-6422
- Persistent URL
- https://folia.unifr.ch/unifr/documents/327804
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