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Pfenninger_et_al_2022_naive
Journal article

Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

DOKPE

  • Pfenninger, Petra ORCID Department of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland
  • Yerly, Laura ORCID Department of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland
  • Abe, Jun ORCID Department of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland
  • 30.06.2022
Published in:
  • Frontiers in Immunology. - 2022, vol. 13, p. 777113
primary CD8+ T cell
CRISPR/Cas9
nucleofection
gene inactivation
target protein depletion
antiviral immunity
CD8+ T cell genetic engineering
English CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8+ T cell biology, it remains elusive to what extent CRISPR/Cas9 affects in vivo function of CD8+ T cells. Here, we optimized nucleofection-based CRISPR/Cas9 genetic engineering of naïve and in vitro-activated primary mouse CD8+ T cells and tested their in vivo immune responses. Nucleofection of naïve CD8+ T cells preserved their in vivo antiviral immune responsiveness to an extent that is indistinguishable from non-nucleofected cells, whereas nucleofection of in vitro-activated CD8+ T cells led to slightly impaired expansion/survival at early time point after adoptive transfer and more pronounced contraction. Of note, different target proteins displayed distinct decay rates after gene editing. This is in stark contrast to a comparable period of time required to complete gene inactivation. Thus, for optimal experimental design, it is crucial to determine the kinetics of the loss of target gene product to adapt incubation period after gene editing. In sum, nucleofection-based CRISPR/Cas9 genome editing achieves efficient and rapid generation of mutant CD8+ T cells without imposing detrimental constraints on their in vivo functions.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Medicine
License
CC BY
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/320460
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