Post-protein binding metal-mediated coupling of an acridine orange-based fluorophore
- Santoro, Giuseppe University of Fribourg
- Blacque, Olivier University of Zurich
- Zobi, Fabio Université de Fribourg
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2012
Published in:
- Metallomics. - 2012, vol. 4, no. 3, p. 253-259
English
The HEW lysozyme (Lys) and the fac-[Re(CO)3(H2O)3]+ complex (1) are used as a simple model system for the description of a new approach to the labelling polypeptides with fluorescent tags. The strategy takes advantage of the reaction of an acridine orange-based fluorophore (AO) with the non-native metal fragment 1 hybridized on the enzyme. A synthetic methodology for the quantitative metallation of the protein is first described and it is then shown that the exogenous metal complex can be exploited for the coupling of the fluorescent probe. All Lys-derived species were characterized by various spectroscopic techniques. It is shown that the approach does not significantly alter the activity of the final fluorescent metallo-protein conjugate (Lys2). The accumulation of Lys2 on Micrococcus lysodeikticus bacteria was observed viaconfocal laser scanning microscopy.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie
- Language
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- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 330355
- DOI 10.1039/c2mt00175f
- Persistent URL
- https://folia.unifr.ch/unifr/documents/309317
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