Journal article

Backbone and side chain NMR assignments of the H-NOX domain from Nostoc sp. in complex with BAY58-2667 (cinaciguat)

  • Makrynitsa, Garyfallia I. Department of Pharmacy, University of Patras, 26504 Patras, Greece
  • Argyriou, Aikaterini I. Department of Pharmacy, University of Patras, 26504 Patras, Greece
  • Dalkas, Georgios Department of Pharmacy, University of Patras, 26504 Patras, Greece
  • Georgopoulou, Dimitra A. Department of Pharmacy, University of Patras, 26504 Patras, Greece
  • Bantzi, Marina Institut für Organische Chemie, Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany - Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland
  • Giannis, Athanassios Institut für Organische Chemie, Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany
  • Papapetropoulos, Andreas Laboratory of Pharmacology, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
  • Spyroulias, Georgios A. Department of Pharmacy, University of Patras, 26504 Patras, Greece
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    30.10.2020
Published in:
  • Biomolecular NMR Assignments. - 2020
English Soluble guanylate cyclase (sGC) enzyme is activated by the gaseous signaling agent nitric oxide (NO) and triggers the conversion of GTP (guanosine 5′-triphosphate) to cGMP (cyclic guanylyl monophosphate). It contains the heme binding H-NOX (heme- nitric oxide/oxygen binding) domain which serves as the sensor of NO and it is highly conserved across eukaryotes and bacteria as well. Many research studies focus on the synthesis of chemical compounds bearing possible therapeutic action, which mimic the heme moiety and activate the sGC enzyme. In this study, we report a preliminary solution NMR (Nuclear Magnetic Resonance) study of the H-NOX domain from Nostoc sp. cyanobacterium in complex with the chemical sGC activator cinaciguat (BAY58-2667). An almost complete sequence-specific assignment of its 1H, 15N and 13C resonances was obtained and its secondary structure predicted by TALOS+.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/309171
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