A simple reaction for dna sensing and chemical delivery

Janett, Elia; Diep, Kim-Long; Fromm, Katharina M.; Bochet, Christian G.

doi:10.1021/acssensors.0c00988

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A simple reaction for dna sensing and chemical delivery

Janett, Elia Department of Chemistry, University of Fribourg, Chemin du Musée 9, CH-1700 Fribourg, Switzerland
Diep, Kim-Long Department of Chemistry, University of Fribourg, Chemin du Musée 9, CH-1700 Fribourg, Switzerland
Fromm, Katharina M. Department of Chemistry, University of Fribourg, Chemin du Musée 9, CH-1700 Fribourg, Switzerland
Bochet, Christian G. Department of Chemistry, University of Fribourg, Chemin du Musée 9, CH-1700 Fribourg, Switzerland

28.08.2020

Published in:

ACS Sensors. - 2020, vol. 5, no. 8, p. 2338–2343

English Reactions templated by nucleic acids are currently at the heart of applications in biosensing and drug release. The number of chemical reactions selectively occurring only in the presence of the template, in aqueous solutions, and at room temperature and able to release a chemical moiety is still very limited. Here, we report the use of the p-nitrophenyl carbonate (NPC) as a new reactive moiety for DNA templated reactions releasing a colored reporter by reaction with a simple amine. The easily synthesized p-nitrophenyl carbonate was integrated in an oligonucleotide and showed a very good stability as well as a high reactivity toward amines, without the need for any supplementary reagent, quantitatively releasing the red p-nitrophenolate with a half-life of about 1 h.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie

Language