Journal article

Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation

  • Thoms, Matthias Biochemistry Centre, University of Heidelberg, Heidelberg 69120, Germany
  • Mitterer, Valentin Biochemistry Centre, University of Heidelberg, Heidelberg 69120, Germany
  • Kater, Lukas Gene Center, University of Munich, Munich 81377, Germany
  • Falquet, Laurent University of Fribourg and Swiss Institute of Bioinformatics, Fribourg 1700, Switzerland
  • Beckmann, Roland Gene Center, University of Munich, Munich 81377, Germany
  • Kressler, Dieter University of Fribourg and Swiss Institute of Bioinformatics, Fribourg 1700, Switzerland
  • Hurt, Ed Biochemistry Centre, University of Heidelberg, Heidelberg 69120, Germany
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    05.10.2018
Published in:
  • Nature Communications. - 2018, vol. 9, no. 1, p. 4094
English During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in which a gently undulating α-helix corresponding to Cgr1 becomes wedged between Rsa4 and the relocated 5S RNP, but the purpose of this insertion was unknown. Here, we show that cgr1 deletion in yeast causes a slow- growth phenotype and reversion of the pre-60S particle to the pre-rotation stage. However, spontaneous extragenic suppressors could be isolated, which restore growth and pre-60S biogenesis in the absence of Cgr1. Whole-genome sequencing reveals that the suppressor mutations map in the Rpf2–Rrs1 module and Rpl5, which together stabilize the unrotated stage of the 5S RNP. Thus, mutations in factors stabilizing the pre-rotation stage facilitate 5S RNP relocation upon deletion of Cgr1, but Cgr1 itself could stabilize the post-rotation stage.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/309094
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