First genomic characterization of blavim-1 and mcr-9-coharbouring enterobacter hormaechei isolated from food of animal origin

Sadek, Mustafa; Nariya, Hirofumi; Shimamoto, Toshi; Kayama, Shizuo; Yu, Liansheng; Hisatsune, Junzo; Sugai, Motoyuki; Nordmann, Patrice; Poirel, Laurent; Shimamoto, Tadashi

doi:10.3390/pathogens9090687

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Journal article

First genomic characterization of blavim-1 and mcr-9-coharbouring enterobacter hormaechei isolated from food of animal origin

Sadek, Mustafa Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, Switzerland - Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83511, Egypt
Nariya, Hirofumi Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, Japan
Shimamoto, Toshi Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, Japan
Kayama, Shizuo Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, Japan
Yu, Liansheng Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, Japan
Hisatsune, Junzo Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, Japan
Sugai, Motoyuki Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, Japan
Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, 1700 Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, 1700 Fribourg, Switzerland - Institute for Microbiology, University of Lausanne and University Hospital Centre, 1011 Lausanne, Switzerland
Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, 1700 Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, 1700 Fribourg, Switzerland
Shimamoto, Tadashi Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, Japan

22.08.2020

Published in:

Pathogens. - 2020, vol. 9, no. 9, p. 687

English We describe here the complete genome sequence of an Enterobacter hormaechei ST279 coharbouring blaVIM-1 and mcr-9 recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible to colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). The antimicrobial susceptibility profile and conjugation experiments were performed. The entire genome was sequenced by the Illumina MiniSeq and Oxford Nanopore methods. The blaVIM-1 and mcr-9 genes are carried on the same IncHI2/pMLST1 plasmid, pMS37a (Size of 270.9 kb). The mcr-9 gene was located within the physical boundaries demarcated by two insertion elements IS903 (upstream) and IS1 (downstream) but did not possess the downstream regulatory genes (qseC/qseB) which regulate the expression of mcr- 9. Therefore, the mcr-9 might be silently disseminated among carbapenem-resistant Enterobacterales. In addition to blaVIM-1 and mcr-9, plasmid pMS37a harbored various antibiotic resistance genes including aac(6’)-Il, ΔaadA22, aac(6’)-Ib-cr, sul1, dfrA1 and tetA. To the best of our knowledge, this is the first report of a blaVIM-1 and mcr-9-coharbouring E. hormaechei isolate of food origin worldwide. The identification of a multidrug-resistant VIM-1 and mcr-9 positive Enterobacter hormaechei isolate from food is worrisome as retail meat and meat products could serve as a vehicle for these MDR bacteria, which could be transferred between animals and humans through the food chain. It further highlights that Enterobacterales co-producing MCR and carbapenemases being found in the food chain indeed correspond to a One- Health issue, highlighting the need for serious steps to prevent their further dissemination.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language