Journal article

Genome-based characterization of two Colombian clinical Providencia rettgeri isolates co-harboring NDM-1, VIM-2, and other β-lactamases

  • Piza-Buitrago, Adriana Bioinformatics Group, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia - Molecular Epidemiology Laboratory, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia
  • Rincón, Verónica Bioinformatics Group, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia - Molecular Epidemiology Laboratory, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia
  • Donato, John Bioinformatics Group, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia - Molecular Epidemiology Laboratory, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia
  • Saavedra, Sandra Yamile Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia
  • Duarte, Carolina Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia
  • Morero, Jaime Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia
  • Falquet, Laurent Biochemistry/Bioinformatics Unit, Université de Fribourg and Swiss Institute of Bioinformatics, Fribourg, Switzerland
  • Reguero, María Teresa Bioinformatics Group, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia - Molecular Epidemiology Laboratory, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia
  • Barreto-Hernández, Emiliano Bioinformatics Group, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia - Molecular Epidemiology Laboratory, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia
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    12.11.2020
Published in:
  • BMC Microbiology. - 2020, vol. 20, no. 1, p. 345
English Providencia rettgeri is a nosocomial pathogen associated with urinary tract infections and related to Healthcare-Associated Infection (HAI). In recent years isolates producing New Delhi Metallo-β-lactamase (NDM) and other β-lactamases have been reported that reduce the efficiency of clinical antimicrobial treatments. In this study, we analyzed antibiotic resistance, the presence of resistance genes and the clonal relationship of two P. rettgeri isolates obtained from male patients admitted to the same hospital in Bogotá – Colombia, 2015. Results: Antibiotic susceptibility profile evaluated by the Kirby-Bauer method revealed that both isolates were resistant to third-generation carbapenems and cephalosporins. Whole-genome sequencing (Illumina HiSeq) followed by SPAdes assembling, Prokka annotation in combination with an in-house Python program and resistance gene detection by ResFinder identified the same six β-lactamase genes in both isolates: blaNDM-1, blaVIM-2, blaCTX-M-15, blaOXA-10, blaCMY-2 and blaTEM- 1. Additionally, various resistance genes associated with antibiotic target alteration (arnA, PmrE, PmrF, LpxA, LpxC, gyrB, folP, murA, rpoB, rpsL, tet34) were found and four efflux pumps (RosAB, EmrD, mdtH and cmlA). The additional resistance to gentamicin in one of the two isolates could be explained by a detected SNP in CpxA (Cys191Arg) which is involved in the stress response of the bacterial envelope. Genome BLAST comparison using CGView, the ANI value (99.99%) and the pangenome (using Roary) phylogenetic tree (same clade, small distance) showed high similarity between the isolates. The rMLST analysis indicated that both isolates were typed as rST-61,696, same as the RB151 isolate previously isolated in Bucaramanga, Colombia, 2013, and the FDAARGOS_330 isolate isolated in the USA, 2015. Conclusions: We report the coexistence of the carbapenemase genes blaNDM-1, and blaVIM-2, together with the β-lactamase genes blaCTX-M-15, blaOXA-10, blaCMY-2 and blaTEM-1, in P. rettgeri isolates from two patients in Colombia. Whole-genome sequence analysis indicated a circulation of P. rettgeri rST- 61,696 strains in America that needs to be investigated further.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308964
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