The type I-E CRISPR-Cas system influences the acquisition of bla KPC-IncF plasmid in Klebsiella pneumonia

Zhou, Ying; Tang, Yu; Fu, Pan; Tian, Dongxing; Yu, Lianhua; Huang, Yunkun; Li, Gang; Li, Meng; Wang, Yong; Yang, Zehua; Xu, Xiaogang; Yin, Zhe; Zhou, Dongsheng; Poirel, Laurent; Jiang, Xiaofei

doi:10.1080/22221751.2020.1763209

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The type I-E CRISPR-Cas system influences the acquisition of bla KPC-IncF plasmid in Klebsiella pneumonia

Zhou, Ying Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China
Tang, Yu Department of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, People’s Republic of China
Fu, Pan Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China
Tian, Dongxing Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China
Yu, Lianhua Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, People’s Republic of China
Huang, Yunkun Department of Laboratory Medicine Kunming Yan’an Hospital, Kunming, People’s Republic of China
Li, Gang Department of Laboratory Medicine, Jinshan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China
Li, Meng Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China
Wang, Yong Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Jinan, People’s Republic of China
Yang, Zehua Department of Laboratory Medicine, Sixth Hospital of Shanxi Medical University, Taiyuan, People’s Republic of China
Xu, Xiaogang Institute of Antibiotics, Huashan Hospital, Fudan University, and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, People’s Republic of China - National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China
Yin, Zhe State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of China
Zhou, Dongsheng State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of China
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - Laboratoire Europeen Associé (LEA) INSERM, IAME (Paris, France), University of Fribourg, Fribourg, Switzerland
Jiang, Xiaofei Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China

20.05.2020

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Emerging Microbes & Infections. - 2020, vol. 9, no. 1, p. 1011–1022

English Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and bla KPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of bla KPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and bla KPC-IncF plasmids in such isolates. We collected 459 clinical K. pneumoniae isolates in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and bla KPC-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR- Cas system in K. pneumoniae could effectively hindered bla KPC-IncF plasmids invasion and existence. Notably, most bla KPC-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of bla KPC in K. pneumoniae populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of bla KPC-IncF plasmids in CG258 K. pneumoniae.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language