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Particle surfaces to study macrophage adherence, migration, and clearance

  • Septiadi, Dedy Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Lee, Aaron Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Spuch‐Calvar, Miguel Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Moore, Thomas Lee Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Spiaggia, Giovanni Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Abdussalam, Wildan Department of High Energy Density, Helmholtz‐Zentrum Dresden‐Rossendorf Bautzner Landstraße 400 Dresden 01328 Germany
  • Rodriguez‐Lorenzo, Laura Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland - Department of Life Sciences, Nano for Environment Unit, Water Quality Group Av. Mestre José Veiga s/n Braga 4715‐330 Portugal
  • Taladriz‐Blanco, Patricia Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4 Fribourg 1700 Switzerland - Department of Chemistry, University of Fribourg Chemin du Musée 9 Fribourg 1700 Switzerland
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    02.07.2020
Published in:
  • Advanced Functional Materials. - 2020, p. 2002630
English Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well‐understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle model, key contributing factors underlying cell adhesion on nonporous silica particle surfaces, migration and engulfment, are simulated and studied. Following a 24 h incubation period, monocyte‐ derived macrophages and A549 epithelial cells are able to adhere and remove particles in their local vicinity through induction of adhesive pulling arise from cell traction forces and phagocytic/endocytic mechanisms, in a size‐dependent manner. It is observed that such particle‐decorated surfaces can be used to address the influence of surface topography on cell behavior. Substrates which presented 480 nm silica particles are able to induce greater development and maturation of focal adhesions, which play an important role in cellular mechanoregulation. Moreover, under a chemotactic influence, in the presence of 30% fetal bovine serum, macrophages are able to uptake the particles and be directed to translocate along a concentration gradient, indicating that local mechanical effects do not substantially impair normal physiological functions.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308734
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