Journal article

Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection

  • Masseron, Amandine Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland
  • Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
  • Falgenhauer, Linda Institute of Medical Microbiology, and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany - Institute of Hygiene and Environmental Medicine, Justus Liebig University Giessen, Giessen, Germany
  • Imirzalioglu, Can Institute of Medical Microbiology, and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany
  • Kessler, Julie Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
  • Chakraborty, Trinad Institute of Medical Microbiology, and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany
  • Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - Institute of Hygiene and Environmental Medicine, Justus Liebig University Giessen, Giessen, Germany - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland
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    01.07.2020
Published in:
  • Diagnostic Microbiology and Infectious Disease. - 2020, vol. 97, no. 3, p. 115059
English OXA-244 is a derivative of OXA-48 showing weaker carbapenemase activity, compromising the detection of corresponding producers in clinical laboratories. Since 2017, the Swiss National Reference Center for Emerging Antibiotic Resistance noticed an increased identification of OXA-244-producing Escherichia coli (n=15) within the country. Different methods (biochemical and immunoassay tests, screening culture media) were tested for the detection of OXA-244 producers. Whole genome sequencing was used to investigate the genetic relatedness between the isolates and the genetic structures at the origin of the acquisition of the blaOXA-244 gene. The mSuperCARBA® medium and the NG-Test CARBA5 assay were found to be suitable tools for detecting all OXA-244-producing isolates. Other selective media did not perform optimally. Among the fifteen strains, five sequence types were identified, with ST38 being predominant. The blaOXA-244 gene was located on the chromosome for all isolates. Overall, detection of OXA-244 producers is challenging and specific guidelines must be followed.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308713
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