Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection

Masseron, Amandine; Poirel, Laurent; Falgenhauer, Linda; Imirzalioglu, Can; Kessler, Julie; Chakraborty, Trinad; Nordmann, Patrice

doi:10.1016/j.diagmicrobio.2020.115059

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Journal article

Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection

Masseron, Amandine Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland
Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
Falgenhauer, Linda Institute of Medical Microbiology, and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany - Institute of Hygiene and Environmental Medicine, Justus Liebig University Giessen, Giessen, Germany
Imirzalioglu, Can Institute of Medical Microbiology, and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany
Kessler, Julie Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
Chakraborty, Trinad Institute of Medical Microbiology, and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Justus Liebig University Giessen, Giessen, Germany
Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - Institute of Hygiene and Environmental Medicine, Justus Liebig University Giessen, Giessen, Germany - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland

01.07.2020

Published in:

Diagnostic Microbiology and Infectious Disease. - 2020, vol. 97, no. 3, p. 115059

English OXA-244 is a derivative of OXA-48 showing weaker carbapenemase activity, compromising the detection of corresponding producers in clinical laboratories. Since 2017, the Swiss National Reference Center for Emerging Antibiotic Resistance noticed an increased identification of OXA-244-producing Escherichia coli (n=15) within the country. Different methods (biochemical and immunoassay tests, screening culture media) were tested for the detection of OXA-244 producers. Whole genome sequencing was used to investigate the genetic relatedness between the isolates and the genetic structures at the origin of the acquisition of the blaOXA-244 gene. The mSuperCARBA® medium and the NG-Test CARBA5 assay were found to be suitable tools for detecting all OXA-244-producing isolates. Other selective media did not perform optimally. Among the fifteen strains, five sequence types were identified, with ST38 being predominant. The blaOXA-244 gene was located on the chromosome for all isolates. Overall, detection of OXA-244 producers is challenging and specific guidelines must be followed.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language