Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation

Cerqueira Campos, Fabiana; Dennis, Cynthia; Alégot, Hervé; Fritsch, Cornelia; Isabella, Adam; Pouchin, Pierre; Bardot, Olivier; Horne-Badovinac, Sally; Mirouse, Vincent

doi:10.1242/dev.186957

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Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation

Cerqueira Campos, Fabiana i, GRe, D (Institute of Genetics, Reproduction and Development), Université Clermont Auvergne - UMR CNRS 6293 - INSERM U1103, Faculté de Médecine, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France
Dennis, Cynthia i, GRe, D (Institute of Genetics, Reproduction and Development), Université Clermont Auvergne - UMR CNRS 6293 - INSERM U1103, Faculté de Médecine, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France
Alégot, Hervé i, GRe, D (Institute of Genetics, Reproduction and Development), Université Clermont Auvergne - UMR CNRS 6293 - INSERM U1103, Faculté de Médecine, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France
Fritsch, Cornelia i, GRe, D (Biology Department), University of Fribourg, Switzerland
Isabella, Adam Committee on Development, Regeneration and Stem Cell Biology, and Department of Molecular Genetics and Cell Biology - The University of Chicago, 920 East 58th Street, Chicago IL 60653, USA
Pouchin, Pierre i, GRe, D (Institute of Genetics, Reproduction and Development), Université Clermont Auvergne - UMR CNRS 6293 - INSERM U1103, Faculté de Médecine, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France
Bardot, Olivier i, GRe, D (Institute of Genetics, Reproduction and Development), Université Clermont Auvergne - UMR CNRS 6293 - INSERM U1103, Faculté de Médecine, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France
Horne-Badovinac, Sally Committee on Development, Regeneration and Stem Cell Biology, and Department of Molecular Genetics and Cell Biology - The University of Chicago, 920 East 58th Street, Chicago IL 60653, USA
Mirouse, Vincent i, GRe, D (Institute of Genetics, Reproduction and Development), Université Clermont Auvergne - UMR CNRS 6293 - INSERM U1103, Faculté de Médecine, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France

01.04.2020

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Development. - 2020, vol. 147, no. 7, p. dev186957

English How extracellular matrix contributes to tissue morphogenesis is still an open question. In the Drosophila ovarian follicle, it has been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils and stress fibers and their respective impact on elongation are unclear. We found that Dystroglycan (Dg) and Dystrophin (Dys) are involved in BM fibril deposition. Moreover, they also orient stress fibers, by acting locally and in parallel to Fat2. Importantly, Dg-Dys complex-mediated cell-autonomous control of F-actin fiber orientation relies on the preceding BM fibril deposition, indicating two distinct but interdependent functions. Thus, the Dg-Dys complex works as a crucial organizer of the epithelial basal domain, regulating both F- actin and BM. Furthermore, BM fibrils act as a persistent cue for the orientation of stress fibers that are the main effector of elongation.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

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