Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model

Magalhães, Joana; Pinheiro, Marina; Drasler, Barbara; Septiadi, Dedy; Petri-Fink, Alke; Santos, Susana G; Rothen-Rutishauser, Barbara; Reis, Salette

doi:10.2217/nnm-2019-0256

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Journal article

Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model

Magalhães, Joana LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal
Pinheiro, Marina LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal - Faculdade de Medicina, Universidade do Porto, Portugal
Drasler, Barbara Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
Septiadi, Dedy Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
Petri-Fink, Alke Chemistry Department, University of Fribourg, Fribourg, Switzerland
Santos, Susana G Instituto de Investigação e Inovação em Saúde, INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal
Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
Reis, Salette LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal

01.02.2020

Published in:

Nanomedicine. - 2020, vol. 15, no. 3, p. 259–271

English Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170–202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro- inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie, AMI - Bio-Nanomatériaux

Language