Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model
- Magalhães, Joana LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal
- Pinheiro, Marina LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal - Faculdade de Medicina, Universidade do Porto, Portugal
- Drasler, Barbara Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
- Septiadi, Dedy Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
- Petri-Fink, Alke Chemistry Department, University of Fribourg, Fribourg, Switzerland
- Santos, Susana G Instituto de Investigação e Inovação em Saúde, INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal
- Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
- Reis, Salette LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal
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01.02.2020
Published in:
- Nanomedicine. - 2020, vol. 15, no. 3, p. 259–271
English
Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170–202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro- inflammatory cytokine (IL-1β, IL-8 and TNF-α) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie, AMI - Bio-Nanomatériaux
- Language
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- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 328522
- DOI 10.2217/nnm-2019-0256
- Persistent URL
- https://folia.unifr.ch/unifr/documents/308615
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