Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa
- Sadek, Mustafa Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland.
- Tinguely, Camille Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland.
- Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
- Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland. patrice.nordmann@unifr.ch. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland. patrice.nordmann@unifr.ch. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. patrice.nordmann@unifr.ch. - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
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21.04.2020
Published in:
- European Journal of Clinical Microbiology & Infectious Diseases. - 2020, vol. 39, no. 9, p. 1657-1662
English
Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually detected by yellow to purple/violet color change of the bromocresol purple pH indicator. The test performance was evaluated by using 50 colistin-resistant and colistin-susceptible P. aeruginosa isolates, among which 10 were colistin resistant. The sensitivity and specificity of the test were found to be 100% (CI95 65.5-100%) and 95% (CI95 81.8-99.1%), respectively, by comparison with broth microdilution as the reference method. The Rapid Polymyxin/Pseudomonas NP test is easy to perform, specific and sensitive, allowing rapid visual observation of results without the requirement any special reading equipment, and results are obtained in 3 h.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 328488
- DOI 10.1007/s10096-020-03884-x
- Persistent URL
- https://folia.unifr.ch/unifr/documents/308537
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