Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa; Tinguely, Camille; Poirel, Laurent; Nordmann, Patrice

doi:10.1007/s10096-020-03884-x

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Journal article

Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland.
Tinguely, Camille Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland.
Poirel, Laurent Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
Nordmann, Patrice Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland. patrice.nordmann@unifr.ch. - INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland. patrice.nordmann@unifr.ch. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. patrice.nordmann@unifr.ch. - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.

21.04.2020

Published in:

European Journal of Clinical Microbiology & Infectious Diseases. - 2020, vol. 39, no. 9, p. 1657-1662

English Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually detected by yellow to purple/violet color change of the bromocresol purple pH indicator. The test performance was evaluated by using 50 colistin-resistant and colistin-susceptible P. aeruginosa isolates, among which 10 were colistin resistant. The sensitivity and specificity of the test were found to be 100% (CI95 65.5-100%) and 95% (CI95 81.8-99.1%), respectively, by comparison with broth microdilution as the reference method. The Rapid Polymyxin/Pseudomonas NP test is easy to perform, specific and sensitive, allowing rapid visual observation of results without the requirement any special reading equipment, and results are obtained in 3 h.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language