How do ethanolamine plasmalogens contribute to order and structure of neurological membranes?

West, Ana; Zoni, Valeria; Teague, Walter E.; Leonard, Alison N.; Vanni, Stefano; Gawrisch, Klaus; Tristram-Nagle, Stephanie; Sachs, Jonathan N.; Klauda, Jeffery B.

doi:10.1021/acs.jpcb.9b08850

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How do ethanolamine plasmalogens contribute to order and structure of neurological membranes?

West, Ana Department of Chemistry, University of Georgia, Athens, Georgia 30602, United States
Zoni, Valeria Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland
Teague, Walter E. Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, NIH, Bethesda, Maryland 20892, United States
Leonard, Alison N. Biophysics Graduate Program, University of Maryland, College Park, Maryland 20742, United States
Vanni, Stefano Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland
Gawrisch, Klaus Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, NIH, Bethesda, Maryland 20892, United States
Tristram-Nagle, Stephanie Biological Physics Group, Physics Department, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States
Sachs, Jonathan N. Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minnesota 55455, United States
Klauda, Jeffery B. Biophysics Graduate Program, University of Maryland, College Park, Maryland 20742, United States - Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, Maryland 20742, United States

06.02.2020

Published in:

The Journal of Physical Chemistry B. - 2020, vol. 124, no. 5, p. 828–839

English Ethanolamine plasmalogen (EtnPLA) is a conical-shaped ether lipid and an essential component of neurological membranes. Low stability against oxidation limits its study in experiments. The concentration of EtnPLA in the bilayer varies depending on cell type and disease progression. Here we report on mixed bilayers of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-(1Z- octadecenyl)-2-oleoyl-sn-glycero-3-phosphoethanolamine (C18(Plasm)-18:1PE, PLAPE), an EtnPLA lipid subtype, at mole ratios of 2:1, 1:1, and 1:2. We present X-ray diffuse scattering (XDS) form factors F(qz) from oriented stacks of bilayers, related electron-density profiles, and hydrocarbon chain NMR order parameters. To aid future research on EtnPLA lipids and associated proteins, we have also extended the CHARMM36 all-atom force field to include the PLAPE lipid. The ability of the new force-field parameters to reproduce both X- ray and NMR structural properties of the mixed bilayer is remarkable. Our results indicate a thickening of the bilayer upon incorporation of increasing amounts of PLAPE into mixed bilayers, a reduction of lateral area per molecule, and an increase in lipid tail-ordering. The lateral compressibility modulus (KA) calculated from simulations yielded values for PLAPE similar to POPC.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language