Journal article

Increased resistance to carbapenems in proteus mirabilis mediated by amplification of the bla vim-1 -carrying and is 26 -associated class 1 integron

  • Bontron, Séverine Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
  • Poirel, Laurent Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
  • Kieffer, Nicolas Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
  • Savov, Encho Military Medical Academy, Sofia, Bulgaria.
  • Trifonova, Angelina Military Medical Academy, Sofia, Bulgaria.
  • Todorova, Iva Military Medical Academy, Sofia, Bulgaria.
  • Kueffer, Gwendoline Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
  • Nordmann, Patrice Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland. - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland. - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
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    30.05.2019
Published in:
  • Microbial Drug Resistance. - 2019, vol. 25, no. 5, p. 663–667
English The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates.Methods: The entire genetic structure surrounding the β- lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing.Results: A series of clinical P. mirabilis isolates were consecutively recovered from different patients at the Military hospital of Sofia, Bulgaria. They showed variable levels of resistance to carbapenems. All isolates produced the same carbapenemase VIM-1 that was chromosomally encoded. We showed that increased resistance to carbapenems was related to an increased number of blaVIM-1 gene copies.Conclusion: We showed here that increased carbapenem resistance in P. mirabilis may result from increased expression of the blaVIM-1 carbapenemase gene through multiplication of its copy number
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308376
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