LCO receptors involved in arbuscular mycorrhiza are functional for rhizobia perception in legumes

Girardin, Ariane; Wang, Tongming; Ding, Yi; Keller, Jean; Buendia, Luis; Gaston, Mégane; Ribeyre, Camille; Gasciolli, Virginie; Auriac, Marie-Christine; Vernié, Tatiana; Bendahmane, Abdelhafid; Ried, Martina Katharina; Parniske, Martin; Morel, Patrice; Vandenbussche, Michiel; Schorderet, Martine; Reinhardt, Didier; Delaux, Pierre-Marc; Bono, Jean-Jacques; Lefebvre, Benoit

doi:10.1016/j.cub.2019.11.038

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LCO receptors involved in arbuscular mycorrhiza are functional for rhizobia perception in legumes

Girardin, Ariane LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France -
Wang, Tongming LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France -
Ding, Yi LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France
Keller, Jean Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Auzeville, BP42617, 31326 Castanet-Tolosan, France
Buendia, Luis LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France
Gaston, Mégane LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France
Ribeyre, Camille LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France
Gasciolli, Virginie LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France
Auriac, Marie-Christine LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France - Institut Fédératif de Recherche 3450, Université de Toulouse, CNRS, UPS, Plateforme Imagerie TRI-Genotoul, 31326 Castanet-Tolosan, France
Vernié, Tatiana Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Auzeville, BP42617, 31326 Castanet-Tolosan, France
Bendahmane, Abdelhafid IPS2, Institute of Plant Science, INRA, Paris-Saclay, 91190 Gif-sur-Yvette, France
Ried, Martina Katharina Genetics, Faculty of Biology, University of Munich (LMU), 82152 Martinsried, Germany
Parniske, Martin Genetics, Faculty of Biology, University of Munich (LMU), 82152 Martinsried, Germany
Morel, Patrice Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France
Vandenbussche, Michiel Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France
Schorderet, Martine Department of Biology, University of Fribourg, Switzerland
Reinhardt, Didier Department of Biology, University of Fribourg, Switzerland
Delaux, Pierre-Marc Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Auzeville, BP42617, 31326 Castanet-Tolosan, France
Bono, Jean-Jacques LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France
Lefebvre, Benoit LIPM, Université de Toulouse, INRA, CNRS, 31326 Castanet-Tolosan, France

16.12.2019

Published in:

Current Biology. - 2019, vol. 29, no. 24, p. 4249-4259.e5

English Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation of LCOs from arbuscular mycorrhizal fungi suggested that LCOs are also signaling molecules in arbuscular mycorrhiza (AM). However, the corresponding plant receptors have remained uncharacterized. Here we show that petunia and tomato mutants in the LysM receptor-like kinases LYK10 are impaired in AM formation. Petunia and tomato LYK10 proteins have a high affinity for LCOs (Kd in the nM range) comparable to that previously reported for a legume LCO receptor essential for the RNS. Interestingly, the tomato and petunia LYK10 promoters, when introduced into a legume, were active in nodules similarly to the promoter of the legume orthologous gene. Moreover, tomato and petunia LYK10 coding sequences restored nodulation in legumes mutated in their orthologs. This combination of genetic and biochemical data clearly pinpoints Solanaceous LYK10 as part of an ancestral LCO perception system involved in AM establishment, which has been directly recruited during evolution of the RNS in legumes.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language