The transcriptional regulator CBX2 and ovarian function: A whole genome and whole transcriptome approach
- Bouazzi, Leila Division of Endocrinology, Section of Medicine, University of Fribourg, Switzerland
- Sproll, Patrick Division of Endocrinology, Section of Medicine, University of Fribourg, Switzerland
- Eid, Wassim Department of Biochemistry, Medical Research Institute, University of Alexandria, Egypt
- Lauber-Biason, Anna Division of Endocrinology, Section of Medicine, University of Fribourg, Switzerland
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19.11.2019
Published in:
- Scientific Reports. - 2019, vol. 9, no. 1, p. 1–18
English
The chromobox homolog 2 (CBX2) was found to be important for human testis development, but its role in the human ovary remains elusive. We conducted a genome-wide analysis based on DNA adenine methyltransferase identification (DamID) and RNA sequencing strategies to investigate CBX2 in the human granulosa cells. Functional analysis revealed that CBX2 was upstream of genes contributing to ovarian function like folliculogenesis and steroidogenesis (i.e. ESR1, NRG1, AKR1C1, PTGER2, BMP15, BMP2, FSHR and NTRK1/2). We identified CBX2 regulated genes associated with polycystic ovary syndrome (PCOS) such as TGFβ, MAP3K15 and DKK1, as well as genes implicated in premature ovarian failure (POF) (i.e. POF1B, BMP15 and HOXA13) and the pituitary deficiency (i.e. LHX4 and KISS1). Our study provided an excellent opportunity to identify genes surrounding CBX2 in the ovary and might contribute to the understanding of ovarian physiopathology causing infertility in women.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 327702
- DOI 10.1038/s41598-019-53370-4
- Persistent URL
- https://folia.unifr.ch/unifr/documents/308335
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