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Detection of HER2+ breast cancer cells using bioinspired DNA-based signal amplification

  • Rafiee, Sarah D. Department of Oncology, Microbiology and Immunology Faculty of Science and Medicine, University of Fribourg Chemin du Musée 18, PER17 1700 Fribourg Switzerland
  • Kocabey, Samet Department of Oncology, Microbiology and Immunology Faculty of Science and Medicine, University of Fribourg Chemin du Musée 18, PER17 1700 Fribourg Switzerland
  • Mayer, Michael Bio, Physics, Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4, PER 18 1700 Fribourg Switzerland
  • List, Jonathan Bio, Physics, Adolphe Merkle Institute, University of Fribourg Chemin des Verdiers 4, PER 18 1700 Fribourg Switzerland - Physics of Synthetic Biological Systems, Technical University Munich Am Coulombwall 4a 85748 Garching Germany
  • Rüegg, Curzio Department of Oncology, Microbiology and Immunology Faculty of Science and Medicine, University of Fribourg Chemin du Musée 18, PER17 1700 Fribourg Switzerland
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    13.01.2020
Published in:
  • ChemMedChem. - 2020, vol. 15, no. 8, p. 661-666
English Circulating tumor cells (CTC) are promising biomarkers for metastatic cancer detection and monitoring progression. However, detection of CTCs remains challenging due to their low frequency and heterogeneity. Herein, we report a bioinspired approach to detect individual cancer cells, based on a signal amplification cascade using a programmable DNA hybridization chain reaction (HCR) circuit. We applied this approach to detect HER2+ cancer cells using the anti‐HER2 antibody (trastuzumab) coupled to initiator DNA eliciting a HCR cascade that leads to a fluorescent signal at the cell surface. At 4 °C, this HCR detection scheme resulted in highly efficient, specific and sensitive signal amplification of the DNA hairpins specifically on the membrane of the HER2+ cells in a background of HER2− cells and peripheral blood leukocytes, which remained almost non‐fluorescent. The results indicate that this system offers a new strategy that may be further developed toward an in vitro diagnostic platform for the sensitive and efficient detection of CTC.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308296
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