Journal article

+ 4 other files

Multilayered control of protein turnover by torc1 and atg1

  • Hu, Zehan Department of Biology, University of Fribourg, Switzerland
  • Raucci, Serena Department of Biology, University of Fribourg, Switzerland
  • Jaquenoud, Malika Department of Biology, University of Fribourg, Switzerland
  • Hatakeyama, Riko Department of Biology, University of Fribourg, Switzerland
  • Stumpe, Michael Department of Biology, University of Fribourg, Switzerland
  • Rohr, Rudolf P. Department of Biology, University of Fribourg, Switzerland
  • Reggiori, Fulvio Department of Biomedical Sciences of Cells & Systems, University of Groningen, University Medical Center Groningen, the Netherlands
  • De Virgilio, Claudio Department of Biology, University of Fribourg, Switzerland
  • Dengjel, Jörn Department of Biology, University of Fribourg, Switzerland
Show more…
    24.09.2019
Published in:
  • Cell Reports. - 2019, vol. 28, no. 13, p. 3486-3496.e6
English The target of rapamycin complex 1 (TORC1) is a master regulator of cell homeostasis, which promotes anabolic reactions and synchronously inhibits catabolic processes such as autophagy-mediated protein degradation. Its prime autophagy target is Atg13, a subunit of the Atg1 kinase complex that acts as the gatekeeper of canonical autophagy. To study whether the activities of TORC1 and Atg1 are coupled through additional, more intricate control mechanisms than simply this linear pathway, we analyzed the epistatic relationship between TORC1 and Atg1 by using quantitative phosphoproteomics. Our in vivo data, combined with targeted in vitro TORC1 and Atg1 kinase assays, not only uncover numerous TORC1 and Atg1 effectors, but also suggest distinct bi-directional regulatory feedback loops and characterize Atg29 as a commonly regulated downstream target of both TORC1 and Atg1. Thus, an exquisitely multilayered regulatory network appears to coordinate TORC1 and Atg1 activities to robustly tune autophagy in response to nutritional cues.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/308288
Other files

Statistics

Document views: 10 File downloads:
  • den_mcp.pdf: 1
  • 1-s2.0-S2211124719311246-mmc1.pdf: 0
  • tbl_s1.txt: 0
  • tbl_s3.txt: 0
  • tlb_s4.txt: 0